竹荪抗氧化物质超声波循环提取工艺

Ultrasonic Circulating Extraction of Total Antioxidant Substances from Dictyophora indusiata

采用新兴的超声波循环萃取技术提取竹荪中抗氧化物质,以DPPH自由基清除率为抗氧化活性指标,通过单因素试验,研究提取温度、超声功率、间歇比、投料量、提取时间和乙醇浓度等6个因素对提取效果的影响。结果表明竹荪中抗氧化物质超声波循环提取的最佳条件为:提取温度40℃,超声功率600W,间歇比3∶1,投料量8%,提取时间15min,乙醇浓度80%。

A new extraction technology-ultrasonic cycling extraction was applied to extract total antioxidant substances from Dictyophora indusiata. The optimal condition was investigated by six single factor experiments, with DPPH radical scavenging ability as antioxidant activity index. The best technical parameters were as follows： the ultrasonic extracting temperature was 40℃, the ultrasonic power was 600W, the intermittent ratio was 3 ： 1 （working 3sec and intermitting lsec）, the material to liquid ratio was 8% （w/v）, the extracting time was 15min, the ethanol concentration was 80 %.