VEGF和透明质酸酶在侵袭性垂体腺瘤中的表达研究

Study of VEGF and hyaluronidase expressions in invasive pituitary adenomas

目的探讨血管内皮细胞生长因子(VEGF)和透明质酸酶在侵袭性垂体腺瘤中的表达及其意义。方法 2004年1月至2010年12月收集手术切除垂体腺瘤标本70例,其中侵袭性垂体腺瘤36例,非侵袭性垂体腺瘤34例,另收集12例正常脑组织标本作为对照。应用免疫组化法检测标本中VEGF蛋白质表达,用逆转录聚合酶链式反应检测标本中VEGF和透明质酸酶基因PH-20 mRNA表达,用酶联免疫吸附法测定标本中透明质酸酶活性。结果在侵袭性垂体腺瘤中VEGF蛋白质和mRNA、透明质酸酶活性及其基因PH-20 mRNA的表达水平分别为(2.52±0.32)分、1.72±0.23、(10.99±3.79)mU/g和1.60±0.28,在非侵袭性垂体腺瘤中分别为(1.56±0.68)分、0.97±0.50、(4.29±1.86)mU/g和0.41±0.19,在正常脑组织中则分别为(0.11±0.09)分、0.08±0.04、(4.10±1.96)mU/g和0.00±0.00。在侵袭性垂体腺瘤组织中VEGF蛋白质和mRNA、透明质酸酶活性及其基因PH-20 mRNA表达均显著高于在非侵袭性垂体腺瘤中的表达(P<0.05),而在非侵袭性垂体腺瘤中的表达又明显高于在正常脑组织中的表达(P<0.05)。结论 VEGF、透明质酸酶与垂体腺瘤的侵袭性密切相关,对侵袭性垂体腺瘤的诊断及预后判断有重要价值。

Objective To investigate the role of vascular endothelial growth factor （VEGF） and hyaluronidase in the invasive biological behavior of pituitary adenoma. Methods The expression of VEGF protein was determined by immunohistochemical technique and the hyaluronidase activity was determined by enzyme linked sorbent assay in 36 specimens of invasive pituitary adenomas, 34 non-invasive pituitary adenomas and 12 normal cerebellar tissues, in which the expressions of VEGF mRNA and Hyaluronidase pH-20 mRNA were detected by reverse transcription-polymerse chain reaction. Results The levels of VEGF protein and mRNA expressions, which were （2.52±0.32） points and 1.72±0.23 respectively, the hyaluronidase activity and the levels of hyaluronidase PH-20 mRNA expressions, which were （10.99±3.79）mU/g and 1.60±0.28 respectively, in the invasive pituitary adenomas were significantly higher than those in the non-invasive pituitary adenomas（P〈0.05）, which were （1.56±0.68） points, 0.97 ±0.50, （4.29± 1.86）mU/g and 0.41 ±0.19 respectively. The levels of VEGF protein and mRNA expressions, and hyaluronidase activity and the level PH-20 mRNA expression in the non-invasive pituitary adenomas were significantly higher than those in the normal brain tissues （P〈0.05）, which were （0.11 ±0.09） points, 0.08±0.04, （4.10± 1.96） mU/g and 0.00±0.00 respectively. Conclusions It is suggested that the VEGF and hyaluronidase are closely related to the invasiveness of the pituitary adenomas, and they have important value to the diagnosis and assessment of the prognoses in the patients with pituitary adenomas.