骨髓间充质干细胞改善糖尿病大鼠勃起功能障碍的研究

Effect of bone marrow mesenchymal stem cells on improving erectile dysfunction in diabetic rats

目的:探讨骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMMSCs)移植到大鼠阴茎海绵体组织后对勃起功能的影响。方法:体外分离培养BMMSCs并利用形态学及流式细胞术进行鉴定证实。用腹腔注射链脲左菌素(Streptozotocin,STZ)建立糖尿病性大鼠模型,并用阿扑吗啡(Apomorphine,APO)筛选出糖尿病勃起功能障碍(Diabetes mellitus induced erectiledysfunction,DMED)大鼠模型,成模后将BMMSCs(2×106个)移植于大鼠阴茎海绵体内。2周后,分别对正常组、糖尿病组及治疗组进行海绵体内压(Intracorporeal pressure,ICP)及平均动脉压(Mean arterial blood pressure,MAP)测定并取大鼠阴茎海绵体组织,荧光显微镜下观察BMMSCs的局部存活情况,苏木素-伊红染色(Hematoxylin and eosin,HE)观察阴茎海绵体组织中血管。结果:荧光显微镜观察提示BMMSCs能在糖尿病大鼠体内存活;勃起功能测定结果表明治疗组大鼠ICP/MAP比值明显高于糖尿病组;HE染色结果显示在治疗组大鼠阴茎海绵体中血管数目[(12.75±1.89)根/HP]多于糖尿病组[(8.05±1.43)根/HP]。结论:BMMSCs能够有效改善糖尿病性大鼠勃起功能。

Objective：To study the effect of bone marrow mesenchymal stem cells（BMMSCs） transplanted into corpus cavernosum on improving erectile dysfunction in diabetic rats. Methods：BMMSCs were separated and cultivated in vitro, then were identified by morphological method and flow cytometry. The diabetic rat model was constructed by intrapertoneal injection of streptozotocin（STZ）, then model of diabetes mellitus induced erectile dysfunction （DMED） was sieved by apomorphine （APO）. BMMSCs （2×10^6 pieces） were transplanted into the corpus cavernosum of the rats in DMED model. Two weeks later,the intracorporeal pressure（ICP） and mean arterial blood pressure（MAP） of normal group, diabetic group and treatment group were determined respectively, the surviving and differentiation of BMMSCs in corpus cavernosum was observed,vessels in corpus cavernosum were counted after staining the tissue by hematoxylin and eosin. Results：The separation and culture of BMMSCs as well as the construction of the rat model with diabetic erectile dysfunction were successful. Many BMMSCs survived in diabetic group under microscope after being transplanted. The results of erectile function test indicated that the ratio of ICP against MAP was higher in treatment group than in diabetic group. There were more vessels in the treatment group[（12.75 ± 1.89） radices/HP] than in diabetic group[（8.05 ± 1.43） radices/HP]. Conclusion： BMMSCs can improve erectile function of diabetic rat.