申克孢子丝菌翻译控制肿瘤蛋白的结构分析与功能预测

Molecular Structure Analysis and Function Prediction on Sporothrix schenckii Translationally Controlled Tumor Protein

【目的】从cDNA文库中识别申克孢子丝菌翻译控制肿瘤蛋白(TCTP)的基因并分析预测其结构功能和应用前景。【方法】利用NCBI和ExPASy网站中的各种基因和蛋白的序列结构信息分析工具,结合DNAstar和VectorNTI suite生物信息学分析软件包,从申克孢子丝菌全长cDNA质粒文库中识别TCTP的基因及其编码区,分析、预测该基因编码的蛋白结构特征和功能。【结果】TCTP基因全长621 bp,编码区具有207个氨基酸。在GenBank同源序列中,其与蓝菌属真菌(Grosmannia clavigera)TCTP氨基酸序列一致性达到78%。理论预测分子质量为64581.4 ku。无质体和线粒体定位序列。预测结果显示编码蛋白含有1个跨膜区、7个亲水性部位和5个主要B细胞表位。与蓝菌属真菌及球毛壳霉(C.globosum)的TCTP进化关系最近。【结论】应用生物信息方法筛选出申克孢子丝菌TCTP的cDNA全长序列并预测其结构与功能、潜在抗原表位,为进一步实验研究该蛋白生物学特性提供了依据。

[ Objective ]The structure and properties about encoding protein of the translationally controlled tumor protein （TCTP） from Sporothrix schenckii were analyzed and predicted by bioinformatics. [ Methods ] A full-length cDNA sequence encoding TCTP from cDNA plasid library of Sporothrix schenckii was identified by using tools of bioinformatics at webs sites of NCBI, ExPASy and software DNAstar, VectnrNTIsuite. The characteristics of the deduced protein were predicted by employing bioinformatics software package supplied by the website of ExPaSy. [Results] A complete open reading frame of 621 bp was included in the full cDNA sequence encoding SS.TCTP. The sequence of TCTP analysis showed that it had high homology in the amino acids sequence from other species in GenBank. The molecular weight of SS.TCTP was predicated to be 64581.4 ku and the coding protein was demonstrated to contain one transmembrane region, three main hydrophilic regions and 5 main B cell epitopes. The relationship of phylogenesis between SS.TCTP and TCTP of other fungus is close. [ Conclusions ] The full-length cDNA sequence encoding TCTP was screened from cDNA library of Sporothrix schenckii bioinformatics and the structure and characteristics of the gene and protein of SS.TCTP as well as the immunological properties were predicted.