sHLA-G对复发性流产患者滋养细胞侵袭功能的影响

Effect and Significance of Exogenous Soluble HLA-G Intervention on Trophoblast Invasion in Patients of Recurrent Spontaneous Abortion

【目的】探讨外源性可溶性人类白细胞相关抗原G(sHLA-G)对不明原因复发性流产(RSA)患者滋养细胞侵袭功能的影响,为探索RSA的有效治疗措施提供依据.【方法】正常组30例,为正常早孕人流患者,无自然流产史,孕周8-10周;RSA组20例,相应孕周RSA患者,绒毛组织20例。滋养细胞原代培养,分离绒毛外滋养细胞,正常组患者每例滋养细胞接种1瓶、RSA患者每例3瓶备实验用。正常组及RSA对照组、研究1组、研究2组分别以生理盐水、生理盐水、5×10-4/L%终浓度sHLA-G、0.1%终浓度sHLA-G干预培养16 h,取悬浮细胞获得绒毛外滋养层细胞(EVCT)团,加入CD146单克隆抗体,采用流式细胞仪检测各组滋养细胞CD146阳性率。【结果】①正常组EVCT CD146阳性率为(63.29±6.76)%,明显高于RSA对照组(10.61±3.34)%,P<0.01。②研究1组EVCT的CD146的阳性率为(30.61±7.52)%,较RSA对照组显著升高,P<0.01;研究2组EVCT的CD146的阳性率为(64.15±9.54)%,显著高于研究1组及RSA对照组,P均<0.01,与正常组比较差异无统计学意义,P>0.05。【结论】外源性sHLA-G干预可显著提高RSA患者EVCT侵袭功能,可望成为RSA的有效治疗措施之一。

[Objective] To study the effect of extrogenous soluble human leukocyte antigen-G （sHLA-G）on trophoblast invasion in chronic villi of unexplained recurrent spontaneous abortion and then provide scientific evidence for treatment strategies. [ Methods ] The villi of 8-10 weeks gestation were collected from 50 women, 30 cases of normal induced abortion, and 20 cases of unexplained recurrent spontaneous abortion （RSA）. Trophoblast cells were primary cultured, 1 bottle for each normal case （normal group） and 3 bottles for each RSA case （control group, group 1, group 2）. 0.9% NS, 0.9% NS, and 0.05%, 0.1% ultimate concentration sHLA-G was added to each group respectively, and continued to cultured for 16 hours. The EVCT were collected from suspension cells and stained by CD146 monoclonal antibody. The expression of CD146 was detected by flow cytometry. [ Results] The rate of CD146± EVCT in normal pregnant group was （63.29 ± 6.76）%, significantly higher than the control group （10.61 ± 3.34）%, P 〈 0.01. （2） The rate of CD146± EVCT was （64.15 ± 9.54）% in group 2 （0.1% sHLA-G intervention）, significantly higher than that of gruopl （0.05% sHLA-G intervention）（30.61 ± 7.52）%,P 〈 0.01. The rate of CD146± EVCT in group 1 and group 2 was both higher than the eontrol group, P 〈 0.01. There was no significant difference between the normal group and group 2. [Conelusion] Exogeneous sHLA-G intervention ean significantly improve the invasion of trophoblast cells from RSA patients. It may be one of the effective treatment strategies for RSA.