SUFR-UCT系统中一株好氧反硝化菌的鉴定与反硝化性能分析

Analysis on denitrification characteristics of an aerobic denitrifier in the system of SUFR-UCT

从螺旋升流式SUFR-UCT系统好氧反应器的活性污泥中分离得到一株好氧反硝化菌Y4,经16SrDNA系列相似性比较和系统发育分析初步鉴定属于Gordonia.sp(戈登氏菌属)。对菌株Y4反硝化能力进行试验研究,结果表明菌株Y4可以在好氧条件下有效去除培养液中的硝酸盐氮,在初始硝酸盐氮质量浓度为286mg/L时,48h脱氮效率可达61.2%。另外试验考察了溶解氧和温度对菌株Y4反硝化效果的影响,结果显示Y4有较高的氧耐受力,在DO为2～11.8mg/L时都可保持较高的脱氮率;菌株Y4对温度适应性强,在30℃时脱氮效率高达90%。试验证明在螺旋升流式SUFR-UCT系统中存在有较好反硝化性能的好氧反硝化菌。

An aerobic denitrifier Y4 is isolated from the activated sludge of UCT system＇s aerobic reactor, which is identified as Gordonia. sp according to the 16S rDNA similarity comparison and analysis. The study on denitrification capability of strain Y4 shows that strain Y4 could effectively remove the nitrate nitrogen in the culture under aerobic conditions, and the nitrogen removal efficiency within 48 hours up to 61.2 % when the initial concentration of nitrate nitrogen is 286 mg/L. Another test investigated the effects of denitrification on DO and temperature, the results show that strain Y4 has a high oxygen tolerance. When the DO is 2--11.8 mg/L, the denitrification rate could be maintained at a high level. Strain Y4 also has a high temperature adaptation, the denitrifying rates is up to 90% at 30 ℃. The tests prove that there is aerobic denitrifier which has good performance of aerobic denitrification in the SUFR-UCT system.