柱前衍生-反相高效液相色谱法测定荔枝果蒂中的氨基酸

Determination of Amino Acids in Pedicel of Litchi Chinensis by AccQ·Tag-High Performance Liquid Chromatography

建立了AccQ·Tag柱前衍生-高效液相色谱法测定荔枝果蒂中氨基酸含量的方法。荔枝果蒂样品在120℃下真空水解22h，再与AQC衍生剂进行衍生，并采用反相液相色谱法-荧光检测器进行分析，外标法计算样品中17种氨基酸的含量。17种氨基酸在35min内可完全分离，荔枝果蒂中氨基酸的含量在2．5-25μmol／L范围内与色谱峰面积呈良好的线性关系，相关系数不小于0．999。方法的加标回收率在71．2％-92．0％之间，测定结果的相对标准偏差为3．16％～9．94％（n=6），方法的检出限（S／N=3）在0．0005-0．0012mg／L之间。

A method for the determination of 17 amino acids in pedicel of Litchi Chinensis was established. The amino acids in pedicel ofLitchi Chinensis were hydrolyzed at 120℃ in the vacuum for 22 h, then the hydrolyte were derivated by AQC reagent and determined by HPLC-FLD. The 17 amino acids were separately within 35 min. The content of amino acids in pedicel of Litchi Chinensis had good relationship in the range of 2.5-25 μ mol / L, the correlation coefficient was not less than 0.999. The recoveries were in the range of 71.2%-92.0%, and the relative standard deviations of determination results were 3.16%-9.94%（n=6）. The detection limits （S/N=3） were 0.000 5-0.001 2 mg/L.