罗格列酮对2型糖尿病模型小鼠学习记忆及中枢免疫炎性反应的影响

Influence of rosiglitazone on learning and memory and central immune inflammatory reaction in Alzheimer disease mice model

目的研究罗格列酮对2型糖尿病模型小鼠(KK小鼠)学习记忆能力及中枢免疫炎性反应的影响。方法将30只KK小鼠随机分为罗格列酮治疗组、空白对照组和模型组。罗格列酮组给药3周,Morris检测各组小鼠的学习记忆能力;眼眶后静脉丛取血,制备血清,检测血清中超氧化物歧化酶(SOD)、丙二醛(MDA)含量;分离海马组织,行免疫组织化学染色检测胶质纤维酸性蛋白(GFAP)的表达;大脑皮层制备组织匀浆,ElISA法检测的介素(IL)-6、IL-1β的含量。结果与模型组比较,罗格列酮组从第4天开始逃避潜伏期明显缩短(P<0.05),跨越平台次数明显增加(P<0.05);血清中SOD含量明显增加(P<0.05),MDA含量明显下降(P<0.05);海马组织GFAP表达明显降低,大脑组织中IL-6、IL-1β含量显著降低(P<0.05)。结论罗格列酮能改善KK小鼠的学习记忆能力,可能与减轻大脑皮层和海马组织的免疫炎性反应和抗氧化应激有关。

Objective To investigate the influence of rosiglitazone on learning and memory and central inmmune inflammatotry reaction in type 2 diabetes mellitus（T2DM）KK mice.Methods The 30 KK mice were randomly divided into the rosiglitazone treatment group,blank control group and model group.The rosiglitazone treatment group was given 3-week medication.The learning and memory ability of each group was detected by Morris water maze examination.blood was collected from the orbital venous plexcus and prepared serum for detecting the activity of superoxide dismutase（SOD） and the content of malondialdbelryde（MDA）.Immunohistochemical staining was employed to observe the glial fibrillary acidic protein（GFAP） in hippocampus.The content of interleukin-6（IL-6） and interleukin-1β（IL-1β） in cerebral cortex homogenate was studied by ELISA.Results The escape latency phase in the rosiglitazone treatment begun from 4 d to be was obviously shortened and the numbers of crossing platform were significantly increased compared with model group（P〈0.05）.The serum content of SOD in the rosiglitazone treatment group was obviously enhanced and the content of MDA was obviously reduced compared with model group（P〈0.05）.The expression of GFAP and the content of IL-6 and IL-1β in the rosiglitazone treatmeat group were obviously desceased compared with the model group（P〈0.05）.Conclusion Rosiglitazone can improve the learning and memory ability of KK mice,which may be related with alleviating the inmmune inflammatory reaction and antioxidative stress.