摘要
对天蓝色链霉菌 1 0 0产蓝色素进行了培养基的优化 ,初步确定了促使蓝色素产率提高的适合生产用的培养基配方 .经过 8d培养 ,蓝色素总色价相对单位可达 2 62 .5U/mL ,比培养基优化前的总色价相对单位 1 60U/mL提高了 64 % .2L罐发酵 6.5d蓝色素总色价相对单位为摇瓶水平的 75% ,但产色素最高峰时间提前了 1 .5d .
The composition of the industrial medium for Streptomyces coelicolor producing blue pigment was studied and optimized. After 8 days fermentation, the total blue pigment relatively yield of 265.2 U/mL was obtained. It is about 64% increasment compared with that from original conditions. After 6.5 days fermentation,the total blue pigment relatively yield from 2 L fermentor is about 75% of that from shaking flask, But the peak production of blue pigment was 1.5 days ahead of that from shaking flask.
关键词
优化
蓝色素
工业化培养基
天蓝色链霉菌
发酵
optimization
blue pigment
industrial medium
Streptomyces coelicolor
