HQT基因在忍冬不同器官中的相对表达量研究

Researches on the Relative Expression of HQT Gene in Different Organs of Lonicera japonica

目的:对忍冬不同器官,即花蕾、叶和茎中HQT(hydroxycinnamoyl CoA quinate hydroxycinnamoyl transfer-ase)基因的相对表达量进行研究,以揭示HQT基因与忍冬绿原酸生物合成之间的相关性。方法:利用半定量RT-PCR方法对忍冬不同器官中的内参Actin基因以及HQT基因进行测定,通过琼脂糖凝胶电泳对PCR结果进行分析。结果:忍冬不同器官中Actin基因条带亮度比较接近,而HQT基因条带亮度差异较大,花蕾中HQT基因条带最亮,叶中次之,而茎中HQT基因条带非常微弱。此结果与绿原酸在忍冬不同器官中的含量相一致。结论:忍冬HQT基因的表达与绿原酸的合成之间可能存在有必然的联系。

Objective：To reveal the correlation between HQT gene and the biosynthesis of chlorogenic acid in Lonicera japonica.Methods：RT-PCR was used to measure the relative expression of HQT gene and reference gene Actin,and agarose gel electrophoresis was used to analyse the PCR results.Results：The brightness of Actin gene strips of different organs was properly similar to each other,but the brightness of HQT gene strips was significantly different.HQT gene strips of alabastrum were the brightest,the brightness of HQT gene strip of leaves took the second place,and the brightness of HQT gene strips of stems was very faint.This result was in accordance with the content of chlorogenic acid in different organs of Lonicera japonica.Conclusion：The expression of HQT gene with the biosynthesis of chlorogenicacid has necessary relation with Lonicera japonica.