南海海洋真菌Penicillium sp. FS60的次级代谢产物研究

Study on Secondary Metabolites of Marine Fungus Penicillium sp. FS60 from the South China Sea

目的:研究南海海洋真菌Penicillium sp.FS60的次级代谢产物及其细胞毒活性。方法:采用正相硅胶柱、反相硅胶柱、凝胶柱、HPLC和薄层制备等色谱技术和重结晶对菌株FS60的发酵产物进行分离纯化,并通过波谱分析进行结构鉴定;以神经胶质瘤细胞SF-268、乳腺癌细胞MCF-7、大细胞肺癌细胞NCI-H460为供试细胞株,采用SRB法测试化合物的细胞毒活性,以金黄色葡萄球菌(SA)、大肠杆菌(EC)和铜绿假单胞菌(PA)为指示菌株,采用改良的MMT法测试化合物的抗菌活性。结果:从发酵物中分离并鉴定了7个化合物,分别为:2,4-二羟基-3,5,6三甲基苯甲酸甲酯(1)、对羟基苯乙酮(2)、5-羟甲基糠酸(3)、isochromophilone VIII(4)、麦角甾醇(5)、过氧化麦角甾醇(6)、啤酒甾醇(7)。结论:化合物1为首次从青霉属真菌中分离得到,化合物3具有较强的抗菌活性,化合物4具有显著的胞毒活性。

Objective：To study the secondary metabolites of the marine fungus Penicillium sp.FS60 from the South China Sea and their cytotoxicities.Methods：The compounds were isolated from the culture of strain FS60 by various chromatographic methods（silica gel,reverse silica gel,Sephadex-LH20,preparative TLC,HPLC and PTLC）and recrystallization.Their structures were identified by extensive analysis of their spectroscopic data.Compounds were tested for their cytotoxicities against SF-268,MCF-7,and NCI-H460 cell lines by SRB method.While,Compounds were tested for their antibacterial activities against S.aureus,E.coli and P.aeruginosa.Results：Seven compounds were isolated from the culture and identified as methyl 2,4-dihydroxy-3,5,6-trimethylbenzoate（1）,4-hydroxyacetophenone（2）,5-hydroxymethyl-furoic acid（3）,isochromophilonesⅧ（4）,ergosterol（5）,ergosterol peroxide（6）,and cerevisterol（7）.Conclusion：Compound 1 is isolated from the genus Penicillium for the first time.Compound 3 is demonstrated to have significant inhibition against S.aureus and P.aeruginosa.Compound 4 is demonstrated to have significant inhibition against the three cell lines.