巴豆油化学成分的GC-MS分析

GC-MS Analysis of Chemical Components in Seeds Oil from Croton tiglium

目的:测定巴豆油的化学成分及含量。方法:巴豆种仁的石油醚提取物经甲酯化或乙醚稀释后,利用气相色谱-质谱法分离巴豆油的化学成分,用面积归一化法处理所得各组分的百分含量。结果:鉴定出17种脂肪酸成分,在酯化后样品中亚油酸、油酸和二十碳一烯酸共占77.33%,为主要组成成分。此外,在乙醚稀释后样品中鉴定出异龙脑、葑醇等5种芳香类化合物。但具致癌及抗艾滋病等作用的佛波醇双酯化合物并未分离获得。结论:中国产巴豆油富含亚油酸、油酸和二十碳一烯酸,但具致癌及抗艾滋病等作用的药效成分含量甚少,采用普通分离提取方法难以获得。

Objective：To identify the chemical components and their relative content in seeds oil from Croton tiglium.Methods：The oil obtained by extracting of the seeds of Croton tiglium with petroleum ether was subjected to methyl-esterification or dilution with ethylether.GC-MS were used to identify the components in croton oil,peak area normalization method was used to determine the relative content of these substances in the sample.Results：Seventeen fat acid components were identified from croton oil.The main components were linoleic acid,oleic acid,and eicosenoic acid in methyl-esterified sample,whose quantities accouted for 77.33%.In addition,five aromatic compounds were also found in the sample diluted with ethylether,such as isoborneol,fenchyl alcohol,etc.Phorbol esters,having carcinogenesis and anti-HIV-1 effects,were not been identified.Conclusion：There are abundant of linoleic acid,oleic acid and eicosenoic acid in the seeds oil extracted from Chinese Croton tiglium.In contrast,the active component with carcinogenesis and anti-HIV-1 might be very rare in the samples and difficult to be obtained by ordinary separating and extracting methods.