甘草糖提取物对小肠上皮细胞迁移及多胺含量影响的研究

Effect of Polysaccharides from Radix Glycyrrhizae on Migration and Polyamines Contents of IEC-6 Cell

目的:观察甘草糖提取物对小肠上皮细胞(IEC-6)迁移及细胞内多胺(腐胺、精脒和精胺)含量的影响。方法:划痕法造细胞损伤后迁移模型,柱前衍生-高效液相色谱法测定细胞内多胺含量。观察甘草糖复合物对细胞迁移及细胞内多胺含量的影响;观察甘草糖复合物对二氟甲基鸟氨酸(DFMO)致细胞迁移抑制和多胺合成抑制的影响。结果:甘草糖提取物(50、100 mg/L)能促进细胞迁移;可逆转由DFMO所致的细胞迁移抑制;可提高细胞迁移过程中细胞内多胺含量;可逆转由DFMO所致的多胺含量降低。结论:甘草对胃肠黏膜损伤的修复作用可能与其提高细胞多胺含量、促进细胞迁移有关。

Objective：To study the effect of polysaccharides from Radix Glycyrrhizae on migration and polyamines（putrescine,spermidine and spermine） contents of IEC-6 cell.Methods：Cell migration model was induced by scratch method in each well,and the polyamines in IEC-6 cell was determined by pre-column derivation high performance liquid chromatography.The polysaccharides inhibited effect on migration and polyamines contents of IEC-6 cells,and on IEC-6 cell migration by DFMO（a polyamines synthesis inhibitor） and the polyamines contents in the cells were observed.Results：The polysaccharides（50 mg/L or 100 mg/L） was able to promote the cell migration,reverse the cell migration inhibition by DFMO,enhance the IEC-6 cell polyamines（putrescine,spermidine and spermine） contents in the process of cell migration and reverse the reduction of polyamines（putrescine,spermidine and spermine） induced by DFMO.Conclusion：The effect of Radix Glycyrrhizae on the gastrointestinal mucosal damage repairing may be related to increasing polyamine content in cells and promoting cell migration.