贵州省1起人间疑似炭疽疫情的病原学诊断与分析

Etiologic diagnosis and analysis on a suspected case of human anthrax in Guizhou

目的从病原学角度判定1起人间疑似炭疽疫情,为疫情的处置提供科学依据。方法采用革兰氏染色、串珠试验、噬菌体实验、生化试验对病人水疱液标本和采集的土壤标本进行炭疽芽孢杆菌培养分离和鉴定,应用Real-time PCR检测致病性基因PA和capA。结果从土壤标本中分离到1株疑似炭疽芽孢杆菌,细菌学方法鉴定为炭疽芽孢杆菌,Real-time PCR检测致病性基因PA和capA均为阳性。结论该起疫情为炭疽芽孢杆菌引起,患者感染为接触炭疽杆菌污染的土壤所致。

Objective To confirm a suspected human anthrax from the aspect of etiology and provide scientific basis for the treatment of anthrax. Methods Conduct bacillus anthracis culture isolation and identification for the vesicular fluid samples of patients and soil samples by gram stain, beaded test, phage test, and biochemi- cal test, and detect the pathogenic genes of PA and capA by real - time PCR test. Results A strain of suspec- ted bacillus anthracis was isolated from the soil sample. It was identified as bacillus anthracis by bacteriologi- cal method, and the pathogenic genes of PA and capA were positive by real -time PCR test. Conclusions The epidemics was caused by bacillus anthracis, and the patients was infected by contacting soil polluted with ba- cillus anthracis.