丹参对大鼠环孢素A慢性肾毒性的保护作用

Prevention of experimental cyclosporin induced chronic nephrotoxicity by radix salviae miltiorrhizae

目的 :为提高同种肾移植术后患者的长期存活率 ,研究丹参对环孢素A (CsA )慢性肾毒性的防护作用。方法 :观察大鼠灌服CsA及低盐饮食 2 8d ,肾功能、病理和转化生长因子 β1(TGF β1)免疫组化的改变及复方丹参注射液对上述改变的防护作用。结果 :CsA能诱导大鼠肾小球滤过率下降 ,增加尿中N 乙酰 β D 氨基葡萄糖苷酶(NAG)的排泄 ,引起肾间质纤维化和小动脉病变等。丹参能改善上述病变 ,减低肾内TGF β1的表达。结论

Aim: To enhance long term success rates for recipients of cadaver kidney transplantation and investigate the effect of radix salviae miltiorrhizae on cyclosporin induced chronic nephrotoxicity. Methods: Rats were on low salt diet and cyclosporine A (CsA) was administered once a day by gastric gavage at doses of 30 mg/(kg·d) for 28 days. Renal functional and morphological changes were assessed. The effects of radix salviae miltiorrhizae on these changes were also evaluated. Results:Characteristic histological changes of proximal tubular injury, tubulointerstitial fibrosis developed in CsA treated rats. They were accompanied by physiological changes of decreased creatinine clearance, impaired renal concentrating ability and increased tubular enzymuria. Radix salviae miltiorrhizae could reduce these changes significantly. Conclusion: Radix salviae miltiorrhizae may be useful for prevention of cyclosporin induced chronic nephrotoxicity.