脾切除对诱发性肝癌大鼠免疫功能的影响

EFFECTS OF SPLENECTOMY ON ANTI TUMOR IMMUNITY DURING THE INDUCTION OF HEPATOCELLULAR CARCINOMA IN RATS

目的 探讨脾切除对诱发性肝癌大鼠抗肿瘤免疫功能的影响。方法 在大鼠诱发肝癌后的第 2和第 4个月时 ,检测脾切除组和对照组大鼠的NK细胞活性、TNF α及淋巴细胞数的变化。结果 脾切除组大鼠NK细胞活性与对照组比较其差异具有显著性意义 (P <0 .0 5 ) ,但外周血淋巴细胞计数、TNF α水平与对照组比较差异无显著性性 (P >0 .0 5 )。结论 脾切除后对诱发性肝癌大鼠的抗肿瘤免疫功能有一定的影响 ,表现为NK细胞活性持续降低 ,而淋巴细胞计数和细胞因子TNF α经过一段时间后可恢复到正常水平 。

Objective To study the effect of splenectomy on the anti tumor immunity in rats with induced hepatocellular carcinoma (HCC). [WT5”HZ]Methods [WT5”BZ]At the second and fourth month of the induced HCC, the NK cell activity, TNF α level and total lymphcyte in blood were measured in the group of splenectomy and the control group. [WT5”HZ]Results [WT5”BZ]There were no different in the total lymphcyte and TNF α in the blood in two groups, but there were significant difference in the NK cell activity between the group of splenectomy and the control group ( P <0.05). [WT5”HZ]Conclusion [WT5”BZ]There are some change in the anti tumor immunity after splenectomy in rats, in which NK cell activity is at low level continuously. TNF α isn′t affected after the second month after splenectomy.