丙酮沉淀法提取杠板归多糖的工艺研究

Study on acetone extraction of the polysaccharide from Polygonum perfoliatum

目的:探讨丙酮沉淀法提取杠板归多糖的最佳工艺条件。方法:以杠板归为研究对象,以水为溶剂提取杠板归多糖,提取液用丙酮进行沉淀,选取离心时间、沉淀时间和沉淀剂用量3个因素做单因素进行正交实验,探索最佳提取条件。结果:丙酮加入量75ml、沉淀时间18h和离心时间18min是丙酮沉淀法提取杠板归多糖的最佳工艺条件。结论:确定了丙酮沉淀法提取杠板归多糖的最佳工艺条件。

Objective： To study the best extraction conditions of polysaccharide from Polygonum perfoliatum by acetone deposition method.Methods： With Polygonum perfoliatum as research object and water as solvent,extraction of tea polysaccharide under certain conditions were precipitated with acetone.The choices level such as centrifugal time,the amount of acetone and precipitation time were made by single factor tests and orthogonal tests.The best extraction conditions were obtained by the experiments.Results： The best extraction conditions of acetone deposition method were： the amount of acetone being 75 ml,centrifugal time being 18 min,precipitation time being 18 h.Conclusion： The best extraction conditions of polysaccharide from Polygonum perfoliatum is determined by acetone deposition method.