摘要
建立实验性自身免疫性肝炎 (EAH)小鼠模型 ,并对其进行动态观察。方法 以同种系S 10 0肝抗原与弗氏完全佐剂充分乳化后两次予小鼠腹腔注射 ,并设单纯磷酸盐缓冲液 (PBS)、S 10 0肝抗原和弗氏完全佐剂处理对照组。将S 10 0肝抗原层析分离后观察自身抗原特异性T细胞增殖反应。结果 模型组小鼠在首次注射后 2周即可见到多形核细胞特别是淋巴细胞的浸润。首次注射后 4周组织学改变达高峰 ,组织学病变的消退较慢 ;血清ALT水平的动态变化与组织学改变相似 ;模型组存在自身抗原特异性T细胞增殖反应。结论 实验性自身免疫性肝炎是一个可能由自身反应性T细胞介导的自身免疫性肝炎的小鼠模型 。
Objective To set up and dynamically observe the mice model of experimental autoimmune hepatitis(EAH).Methods The syngeneic S 100 hepatic antigen emulsified in complete Freud's adjuvant(CFA) was injected intraperitoneally at day 0 and day 7, and compared with groups treated with PBS, S 100 hepatic antigen or CFA solely. The S 100 antigen was separated by a 90cm CL 6B sepharose colum cell proliferation.Results Infiltrates of polymorphonuclear leukocytes with some lymphocytes could be seen which were induced by intraperitoneal immunization with S 100 proteins in CFA within 2 weeks after the first immunization. The liver injury reached its peak at 4 weeks after the first immunization and resolution of hepatitis was slow. The dynamic change of serum ALT level was similar to that of the pathologic lesions. Specific T cell proliferative activity against fractions of S 100 could be demonstrated in vitro.Conclusion EAH is a murine model of autoimmune hepatitis probably mediated by autoreactive T cells which can be used in the study of the pathogenesis of autoimmune hepatitis.
出处
《肝脏》
2000年第2期70-71,88,共3页
Chinese Hepatology
