细胞水化状态对鼠肝细胞DNA代谢的调节

Hepatocellular hydration state on the modulation of DNA metabolism in rat hepatocyte

探讨不等渗培养条件肝细胞水化状态 (即容积 )改变对鼠肝细胞DNA合成代谢的影响。方法 动态观察 [3H]TdR、[3H]亮氨酸掺入量及培养肝细胞上清液中ALT ,AST ,LDH活性。结果 培养 12 0min ,与等渗组比较 ,[3H]TdR和 [3H]亮氨酸掺入量在高渗组分别减少 6 5 .70 %和 72 .99% (P <0 .0 0 1) ,低渗组减少 15 .94%和 10 .41% (P >0 .0 5 )。 3种渗透状态下两者有非常显著正相关 (P <0 .0 0 1)。同期培养上清液ALT、AST、LDH活性在正常范围内波动。结论 肝细胞水化状态对DNA合成与蛋白合成的调节趋势相近 ,该调节是肝细胞对环境变化的生理反应之一 。

Objective To investigate the metabolic changes of DNA synthesis induced by the change of hydration status within hepatic cells under aniso osmotic conditions.Methods Incorporation rates of leucin into protein and TdR into DNA and the activities of ALT、AST and LDH in the supernatant of cell culture were observed. Results The cells were cultured for 120 minutes, the incorporation rates of leucine and TdR in the hepatocytes of the hyper osmotic group decreased 72.99% and 65.70% respectively as compared with those of the normo osmotic group( P <0.001), and they were 15.94% and 10.41% in the hypo osmotic group ( P>0.05 ). The two incorporation rates were highly correlated in all the hypo 、hyper and normo osmotic groups. The activities of ALT、AST and LDH in the supernatant of cell cultures were within normal range.Conclusions These results indicate that the change of hepatocellular hydration status can regulate the DNA and the protein synthesis. This regulation may be one of the physiological factors to modulate cellular metabolism and the tolerance of hepatocytes to hypo osmotic environment which is greater than that to hyper osmotic one.