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IL-1β对培养大鼠胆碱能神经元的影响及机制探讨 被引量:3

Study on the effect and mechanism of IL-1β on cultured cholinergic neuron in the rat
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摘要 目的 :探讨IL - 1β对大鼠胆碱能神经元的影响及机理。 方法 :检测不同时相、不同脑区的培养神经细胞AChE、BuchE及ChAT的活性和前脑基底、皮层中胆碱酯酶阳性细胞数目。结果 :IL - 1β对AChE活性的最大促进作用时相为 2 4h ,皮层、前脑基底和海马部位的AChE活性明显高于对照组 (P <0 .0 1) ,且能够完全被IL -1ra所阻断 ;而小脑、脊髓、基底神经节部位的AChE活性则未见明显变化 ,同时各组BuchE、ChAT的活性均无明显变化 ,前脑基底、皮层中胆碱酯酶阳性细胞数目亦未见明显变化。结论 :IL - 1β能够明显升高特定脑区AChE活性 ,使乙酰胆碱的生成减少 ; AIM: The effect and mechanism of IL-1β on cultured cholinergic neuron in the rat was studied . METHODS: The acetylcholinesterase (AChE), choline acetyltransferase (ChAT), butyrylcholinesterase (BuchE) activities in different time-phase and different parts of brain were measured. The count number of positive AChE cell in basal forebrain and cortex was also measured. RESULT: It proved that the maximum effect of IL-1β on AChE activity is 24 hour. The AChE activity increased significantly in cortex, basal forebrain and hippocampus, comparing with control ( P< 0.01, P< 0.01, P< 0.05), and its effect can be blocked completely by IL-1ra;but there is no change of AChE activity in cerebellum, spinal and basal ganglia. In the same time, the activity of BuchE and ChAT has no change in every group. It was also found that there is no difference in percent of positive AChE cell in cortex and basal forebrain cell . CONCLUSION: IL-1β can only increase the AChE activity of specific parts in brain so that the synthesis of acetylcholine decrease. The increasing activity of AChE maybe related to the transcription and translation of AChE gene.
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2000年第7期619-622,共4页 Chinese Journal of Pathophysiology
关键词 大鼠 老年性痴呆 IL-1Β 胆碱能神经元 Interleukin-1 Acetylcholinesterase Choline acetyltransferase Cells, culture Rat
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