补肾活血中药对免疫介导再障小鼠骨髓CD_(45)^+细胞的影响

Study on efficacy of the decoction of invigorating kidney and activating blood circulation in BALB/C mice with immune mediated aplastic anemia

目的 :观察补肾活血中药对免疫介导再障小鼠CD+4 5细胞的影响。方法 :建立免疫介导再障小鼠模型 ,随机分组胃饲生理盐水、高剂量中药、低剂量中药、环孢素A(CsA) ,并设正常对照 ,第 10d分别称体重、计算外周血细胞和骨髓有核细胞数、采用流式细胞技术测定骨髓CD+4 5细胞。结果 :中药组小鼠体重、外周血细胞、骨髓有核细胞数、骨髓CD+4 5细胞显著高于空白模型组和CsA组 ,而接近正常对照组。结论

AIM and METHODS: To study the efficacy of the decoction of invigorating kidney and activating blood circulation(DIKABC) in BALB/C mice with immune mediated aplastic anemia. 50 female BALB/C mice at 8～12 weeks of age were divided into 5 groups:(1) control,(2) model,(3) model+DIKABC(H), (4) model+DIKABC(L), and (5) model+ciclosporin A(CsA). The model of immune mediated aplastic anemia in BALB/C mice, which received sublethal whole body irradiation, lymph node and thymus cells of DBA/2 mice, was given different liquid from groups and administered appropriately for 9 days, blood cells and bone marrow nucleated cells(BMNC) and CD + 45 cells were determined by flow cytomytry(FCM).RESULTS: RBC,WBC,Platelet,BMNC in DIKABC groups were higher than those in model group ( P <0.01/0.05), but those in CsA group were lower than those in DIKABC groups ( P <0.05).Compared with model group, CD + 45 cells in DIKABC groups increased ( P <0.05),but were lower than those in control groups ( P< 0.05). CONCLUSION: The decoction of invigorating kidney and activating blood circulation could increase the percentages of CD + 45 cells in bone marrow and prevent immune mediated aplastic anemia in BALB/C mice.