糖尿病大鼠膈肌微量元素与氧化应激变化的研究

Study on the variation of trace elements and oxidative stress in diaphragm of the diabetic rats

目的 :了解糖尿病膈肌微量元素与氧化应激的关系。方法 :采用原子吸收光谱法 ,测定糖尿病 2 8d大鼠膈肌组织中铜 (Cu)、锰 (Mn)锌 (Zn)、铁 (Fe)、镉 (Cd)、铬 (Cr)、钴 (Co)、钼 (Mo)和锂 (Li)的含量 ,并同时测定超氧化物歧化酶 (SOD)活性和丙二醛 (MDA)含量。结果 :糖尿病膈肌Cu、Zn、Cr、Li含量明显低于对照组 ,Fe和Cd明显高于对照组 ,而Mn、Co、Mo与对照组无明显差别。糖尿病膈肌组织SOD活性明显低于对照组 ,MDA含量明显高于对照组。糖尿病膈肌组织Zn含量与其SOD活性呈正相关 ,与MDA含量呈负相关 ,Cd含量与SOD活性呈负相关。结论 :糖尿病 2 8d膈肌组织存在Cu、Zn、Fe、Cd、Cr、Li含量的变化及氧化应激增加 ,Zn和Cd在糖尿病膈肌组织氧化应激变化中具有重要意义。

AIM:To investigate the relationship between trace elements and oxidative stress in diabetic diaphragm. METHODS: Contents of copper(Cu), manganese(Mn), zinc(Zn), iron(Fe),cadmium(Cd),chromium(Cr),cobalt(Co),molybdenum(Mo) and lithium(Li) in the alloxan-induced diabetic rat diaphragm muscles were measured by atomic absorption spectrophotometer, and their superoxide dismutase(SOD) activities and malondiadehyde(MDA) contents were detected. RESULTS: The contents of Cu, Zn, Cr and Li were lower and the Fe and Cd were higher in diabetic rat diaphragm muscles than that of the control group, but there were no significant differences in Mn, Co and Mo, between control and diabetic group. SOD activities decreased significantly and MDA contents increased significantly in diaphragm of diabetic rats as compared with the control group.In addition,in diabetic rats diaphragm, there was a positive correlation between Zn and SOD, and negative correlation between Zn and MDA, and Cd and SOD. CONCLUSION: Changes of the contents of Cu, Zn, Fe, Cd, Cr, Li and the increase of oxidative stress in diaphragm were found in the early diabetes, and Zn, Cd play an importment role in oxidative stress of diabetic diaphragm.