慢性阻塞性肺疾病细菌生物膜检测及耐药性分析

Study on the Determination and Drug Resistance of Bacteria Biofilm in Chronic Obstructive Pulmonary Disease

目的研究慢性阻塞性肺疾病（COPD）患者细菌生物膜检测及耐药机制。方法100例慢性阻塞性肺疾病（c0PD）住院患者痰液标本采用刚果红培养法，检测细菌生物膜形成，K—B琼脂扩散法进行抗菌药敏试验。结果检测到G＋细菌中14株产生生物膜（阳性率58．3％），其中金黄色葡萄球菌9株，表皮葡萄球菌5株；G-细菌中10株产生生物膜（阳性率17．3％），其中普通变形杆菌6株，铜绿假单孢菌4株；产生生物膜的葡萄球菌呈多重耐药，对复方新诺明耐药明显较重，且高于无生物膜葡萄球菌（x2=4．03，P〈o．05）；有生物膜的铜绿假单孢菌／普通变形杆菌对哌拉西林和阿米卡星的耐药率明显比无生物膜的高（x2=6．43，P〈0．05）。结论细菌生物膜形成的实验室检查对COPD患者临床抗生素的使用有极大帮助。

Objective To acquire the bacteria biofilm determination and drug resistance. Methods Biofilm-forming ability was determined by congo red agar and drug resistance test by K-B method. Results The positive rate of bifilm-forming in G＋ and G- bacteria were 88.9% and 17.3% respectively. Biofilm-staphylococcus was drug resistance to trimesulf（x2 =4.03, P 〈0. 05）,susceptibleto vancomycin. Biofilm-peseudomonas aeruginosa/proteus were susceptible to Piperaeillin and amikacin （X2 =6.43, P〈0. 05）. Conclusion Biofilm or no biofilm-bacteria should been identified. Compound antibiotics had effection on biofilm-bacteria of chronic obstructive pulmonary disease.