并殖吸虫病患者血清IL-5、IL-4、IL-13及胸腺基质淋巴细胞生成素水平检测

Detection on serum levels of IL-5, IL-4, IL-13 and thymic stromal lymphopoietin in paragonimiasis patients

目的研究并殖吸虫感染者Th2免疫应答产生的细胞因子IL-5、IL-4、IL-13、胸腺基质淋巴细胞生成素（thymiestromallymphopoietin，TSLP）的水平，筛选出可以用于临床诊断并殖吸虫病的潜在细胞因子标志物。方法收集贵州省本地并殖吸虫病患者成年组（年龄〉18周岁）及未成年组患者（年龄≤18周岁）的血清样本各12例，以健康人血清4例为对照，ELISA检测血清中的IL-5、IL-4、IL-13、TSLP等4种细胞因子的浓度，各病例组及对照组间采用独立样本t检验分析，同时收集患者的流行病学信息。结果成年组IL-5、IL-4、IL-13、TSLP分泌浓度分别为（19．50±5．57）pg／ml、（0．954-0．19）pg／ml、（475．30±629．81）pg／ml、（16676．67±7169．29）pg／ml；未成年组分别为（23．04±3．37）pg／ml、（1．05±0．19）pc,／ml、（422．84±539．48）pc,／ml、（16242．50±6230．81）pg／ml；对照组分别为（4．45±0．84）pg／ml、（0．32±0．12）pg／ml、（41．15±11．72）pg／ml、（490．00±123．36）pg/ml。IL．13、TSLP在成年组、未成年组患者中升高，具有统计学意义（f=1．27，P〈0．05；t=3．11，P〈0．05）。在有典型并殖吸虫病流行病学特征的未成年组中，IL-5升高（t=3．11，P〈0．01），具有统计学意义；而在无典型症状的成年组中的变化无统计学意义（t=1．52，P〉0．05）。IL-4在成年组、未成年组患者中均无统计学意义变化（t=1．08，P〉0．05；t=2．1，P〉0．05）。结论并殖吸虫病患者Th2免疫反应活化，IL-5、IL-13、TsLP显著上调，可以作为潜在的诊断标志物进行下一步的研究筛选。

Objective To investigate the cytokines including IL-5, IL-4, IL-13 and thymic stromal lymphopoietin （TSLP） levels of Th2 immune response to Paragonimus infections, thus to find the potential biomarkers for clinical diagnosis on parasitic worms infections. Methods The sera of local paragonimiasis patients from adult （ 〉 18 years old） and juvenile （ ≤ 18 years old） group in Guizhou Province were collected, respectively. The concentration of IL-5, IL-4, IL-13 and TSLP was measured by ELISA and analyzed with independent sample t test among each patient group and control group. Simultaneously the epidemical data of patients were investigated. Results The concentration of IL-5, IL-4, IL-13 and TSLP in adult group was （9.50 ±5.57） pg/ml, （0.95 ±0.19） pg/ml, （475.30 ±629.81） pg/ml and （16 676.67 ±7 169.29） pg/ml, respectively while in juvenile group was （23.04 ± 3.37） pg/ml, （ 1.05 ±0. 19） pg/ml, （422.84 ±539.48） pg,/ml and （16 242.50 ±6230.81 ） pg/ml, respectively. Meanwhile the concentration in control group was （4.45±0.84） pg/ml, （0.32± 0.12） pg/ml, （41. 15 ± 11.72） pg/ml, （490.00±123.36） pg/ml, respectively. IL-13 and TSLP increased significantly in two patient groups （ t = 1.27, P 〈 0. 05 ; t = 3. 11, P 〈 0. 05 ）. IL-5 increased significantly in juvenile with typical paragonimiasis epidemiology features （ t = 3. 11,P 〈0.01 ）. On the contrary, IL-5 had no significant change in adults without typical symptoms （ t =1. 52, P 〉 0.05）. The variation of IL-4 in two patient groups had no statistical significance （ t = 1.08, P 〉 0. 05 ; t = 2.1, P 〉 0.05）. Conclusion Th2 immune response was activated in paragonimiasis patients with significant up regulation of IL-5, IL-13, and TSLP. The cytokines are of worth to be further researched and screened as potential biomarkers.