微囊化小肽对刺参免疫酶活力的影响

Effects of Microencapsulation of Small Peptide on the Activities of Immunoenzymes in Apostichopus Japonicus

利用微胶囊技术对小肽进行包埋,制成不溶于水的微囊化小肽投喂刺参。根据水溶性肽测定方法,测得微囊化小肽的载药量为21.0%。研究了口服不同剂量微囊化小肽对刺参酸性磷酸酶(ACP)、碱性磷酸酶(AKP)和超氧化物歧化酶(SOD)活力的影响。在基础饲料中分别添加微囊化小肽1.0%、3.0%和空白微胶囊(不含小肽)1.0%、3.0%,以基础饲料饲喂组为对照,饲喂7d。于投喂结束后第2、7和14天检测ACP、AKP与SOD活力。结果显示,投喂空白微胶囊组的酶活力与对照组差异不显著(P>0.05),而投喂微囊化小肽1.0%、3.0%组的酶活力均显著提高,与对照组相比存在显著差异(P<0.05),且微囊化小肽3.0%组的效果最优,其ACP、AKP与SOD活力分别在投喂后第7、7、2天达到最高值,是对照组的190%、380%、210%。解决了刺参摄食较慢,小肽较易溶于水而无法用传统方法给药的问题,有效增强了刺参机体的免疫力。研究结果表明,微囊化小肽可以作为刺参免疫增强剂使用,有成为刺参饲料添加剂的前景。

In this study, the immunostimulatory effect of microencapsulation ol small peptme on me acuvlties of immunoenzymes in apostichopus japonicus. The drug loading of microencapsulated small peptide was up to 21.0% according to the determination method of water-soluble peptide. The basal diet was added 1.0%, 3.0% microencapsulated small peptide and 1.0%, 3.0% blank microcapsules（without small peptide）, the control group was fed the basal diet only for 7 days. The activities of acid phosphatade（ACP）, alkaline phosphatase（AKP） and superoxide dismutase（SOD） in its eoelomie fluid were screened on the 2nd, 7th and 14th day after the end of feeding. The results exhibited that the groups supplemented blank microcapsules had no significant differences in immunoenzyme activities relative to that of the control group. The activities of the groups fed mieroencapsulated small peptide were improved and obviously higher than that of the control（P〈0.05）. A diet supplemented with 3.0% microencapsulated small peptide appeared to have the most effect on immunoenzyme activities in apostichopus japonicus. Its activities of ACP, AKP and SOD were up to maximum value on the 7th, 7th and 2nd day respectively. The values were 190%, 380% and 210% as high as that of the control respectively. In conclusiop, this study solves the problem that we can not use the traditional method to add small peptide. Microencapsulation of small peptide can improve the non-specific immunity level of apostichopus japonicus and has the prospect as a feed additive.