和胃安神方对失眠模型大鼠血浆及下丘脑胆囊收缩素8的影响

Effect of Hewei Anshen Decoction on cholecystokinin-8 levels in plasma and hypothalamus of insomnic rats

目的探讨和胃安神方对失眠大鼠血浆及下丘脑胆囊收缩素8(CCK-8)的影响。方法将30只雄性wistar大鼠随机分为正常对照组、失眠模型组、和胃安神方中药组。采用放射免疫分析法检测血浆及下丘脑组织CCK-8的含量。结果和胃安神方中药组血浆CCK-8含量为(19.320±4.577)pg/ml,高于模型组(12.924±5.132)pg/ml,差异有统计学意义(P<0.05),与正常组(23.718±6.917)pg/ml比较无显著性差异(P>0.05);和胃安神方中药组下丘脑CCK-8含量为(1.655±0.415)pg/mg,高于模型组(1.058±0.398)pg/mg,差异有统计学意义(P<0.05),与正常组(1.936±0.652)pg/mg比较无显著性差异(P>0.05)。结论和胃安神方治疗失眠症的疗效机制可能与其上调CCK-8水平有关。

Objective To study the effect of Hewei Anshen Decoction （HWASD） on CCK-8 levels in plasma and hypothalamus of insomnic rats. Methods Thirty healthy male wistar rats were randomly divided into three groups：normal control group,insomnia model group and HWASD treatment group. Radio- immunoassav was used to measure the levels of CCK-8 in plasma and hypothalamus. Results The concentration of CCK-8 in the plasma of HWASD treatment group was （ 19. 320 ± 4. 577 ） pg/ml, significantly higher than that of insomnia model group （ 12. 924±5. 132） pg/ml （ P〈0.05 ）, similar to normal control group （23. 718±6. 917） pg/ml （P〉0.05）. The level of CCK-8 in the hypothalamus of HWASD treatment group was （ 1. 655 ±0. 415 ） pg/mg, significantly higher than that of insomnia model group（ 1. 058±0. 398） pg/mg （ P〈0.05 ）, similar to normal control group （ 1. 936-0. 652） pg/mg （ P〉 0. 05 ）. Conclusion The therapeutic effect of Hewei Anshen Decoction on insomnia is partially attributed to increased content of CCK-8.