广州地区非洲籍群体17个Y-STR基因座遗传多态性及群体遗传分析

Genetic polymorphism of 17 Y-STR loci in Guangzhou African population and analysis of population genetic distance

目的:研究广州地区居住的非洲籍男性无关个体的17个Y染色体上的短串联重复序列(Y-STR)基因座遗传多态性,为法医学DNA检验、鉴定提供基础数据并进行群体遗传分析。方法:使用AmFISTR YfilerTM试剂盒对104份广州地区的非州籍男性无关个体样本进行检测,获得以上样本DYS456,DYS389Ⅰ,DYS389Ⅱ,DYS390,DYS458,DYS19,DYS385a/b,DYS393,DYS391,DYS439,DYS635,DYS392,GATA_H4,DYS437,DYS438,DYS448等17个Y-STR基因座的遗传资料,并与中国汉族和国外有关群体进行群体遗传距离比较,并绘制遗传距离多维尺度分析图。结果:在104个男性无关个体样本中,共检验出98种单倍型,其中有5种单倍型出现了2次,单倍型多样性达0.9991。广州非洲籍群体群体与中国汉族遗传距离RST值最大为0.3576,与纳米比亚人群RST值最小为0.0512。结论:AmFISTR YfilerTM试剂盒所包含的17个Y-STR基因座,在广州地区非洲籍男性群体中具有较高的遗传多态性,所获得的Y-STR数据,在对该群体的法医学个体识别、父权鉴定以及群体遗传分析中具有重要的指导意义。

Objective：To investigate the genetic polymorphisms of 17 Y-chromosomal short tandem repeatsauthor（STR） loci in Guangdong African population,provide basic statistics data for forensic application and to analyze the genetic relationships with 4 other populations.Methods：Seventeen Y-STR loci（DYS456,DYS389I,DYS390,DYS389 II,DYS458,DYS19,DYS393,DYS385a/b,DYS391,DYS439,DYS635,DYS392,GATA_H4,DYS437,DYS438,DYS448） were detected from 104 unrelated Guangzhou African male individuals by AmFISTR YfilerTM kit.We compared the above genetic information with Chinese Han population and three other African populations.Results：A total of 98 haplotypes were identified,5 of which were found twice.The overall haplotype diversity for the 17 Y-STR loci was 0.9991.Compaed with 4 other populations,the genetic distance between Guangzhou African population and Chinese Han population was the largest（0.3576）,while that between Guangzhou African population and Namibia population was the smallest（0.0512）.Conclusion：Multiplex detection of the 17 Y-STR loci by AmFISTR YfilerTM kit revealed a highly genetic polymorphsm.The Y-STR data investigated in this study would be useful in individual and fatherhood identification as well as population genetics analysis.