废弃菌包中纤维素分解菌的分离与筛选

Isolation and Screening of the Abandoned Bacteria Package Cellulose Decomposing Bacteria

从废弃菌包中分离得到的11株菌株通过固体培养初筛和摇瓶发酵复筛,筛选出纤维素酶活性较高的2株菌株,分别记为f-6和f-10菌株。f-6菌株的Cx酶活力最高,作用于可溶性纤维素的效率最高;f-10菌株的复合酶活力最高,作用于不溶性纤维素的效率最高。在实际应用中,作用于可溶性的纤维素应该考虑选用菌株f-6,作用于不溶性的纤维素则考虑选用菌株f-10。

2 out of 11 strains were be isolated from the waste bacteria package by the solid culture and shake-flask fermentation. These two strains were included into the f-6 and f-10 strains, especialy Cx enzyme of f-6 strain was the most active one,which could be act on the soluble cellulose in the most efficiente, the f-10 strain was the most efficitive enzyme on the insolatle cellulose.In practical applications. It should be take consider into f-6 to, act on soluble cellulose, at the same time, and into f-10 to act on in- soluble cellulose.