新疆沙枣总黄酮含量测定及提取工艺优化

Determination of Total Flavonoids in Elaeagnus angustifolia from Xinjiang and Optimization of the Extraction Process

以芦丁为对照品,NaNO2-Al(NO3)3-NaOH显色体系为显色剂,采用分光光度法测定新疆沙枣(E-laeagnus angustifolia L.)果实、树叶和树枝中总黄酮的含量。采用正交试验优化新疆沙枣树叶中总黄酮的提取工艺。结果表明,新疆沙枣树叶的总黄酮含量为(18.75±0.11)mg/g(n=3),树枝和果实的总黄酮含量分别为(11.72±0.12)mg/g和(8.75±0.10)mg/g(n=3)。新疆沙枣树叶总黄酮的最佳提取工艺为乙醇体积分数70%,提取时间1.5 h,料液比1∶12(m/V,g∶mL),提取2次。该方法简单、准确、重复性好。

Total flavonoids in fruit, leaves and branch of Elaeagnus angustifolia L. was determined by spectrophotometry with rutin as standard agent and NaNO2-A1 （NO3）3-NaOH system as chromogenic reagent. Orthogonal test was adopted to study the extraction process in leaves. The content of total flavonoids in leaves, branch and fruit was （18.75±0.11）,（11.72±0.12）,（8.75±0.10）mg/g （n=3）, respectively. The optimum extraction technology of total flavonoids from E. angus tifolia leaves was, volume fraction of alcohol, 70%; extraction time, 1.5 h; material to liquid ratio, 1：12 （m/V,g：mL）; extraction time, twice. The method was simple, accurate and repeatable.