AM真菌对烟苗生长及叶绿素荧光特性的影响

Effects of Arbuscular Mycorrhizal Fungi on Growth and Chlorophyll Fluorescence Characteristics of Tobacco Seedlings

为了探明不同AM真菌对烟苗生长的作用效果,通过在烟草播种期接种7种不同AM真菌研究其对烟苗生长及叶绿素荧光特性的影响。结果表明:1)接种164菌根烟苗的生物量和171菌根烟苗的最大叶面积显著高于无菌根烟苗。2)菌根烟苗的株高和主根长均显著低于无菌根烟苗,根冠比变化不明显;菌根烟苗的叶绿素a/b和类胡萝卜素含量显著提高,而总叶绿素含量、叶绿素a含量和叶绿素b含量只有接种Gm01、Gm02、186的菌根烟苗显著高于无菌根烟苗。3)接种AM真菌对烟苗叶片叶绿素荧光特性的影响较大,菌根烟苗的PSII实际光合效率、光化学淬灭系数、最大相对电子传递速率、半饱和光强均高于无菌根烟苗,调节性能量耗散的量子产额及非调节性能量耗散的量子产额降低,即菌根烟苗对强光的耐受能力更强,能较好地利用红光促进烟苗生长,但不同AM真菌的菌根效应不同。

In order to probe into the effects of different AM fungus on growth of tobacco seedlings, effects of AM fungus on growth and chlorophyll fluorescence characteristics of tobacco seedlings were studied by tobacco seeds inoculated with seven kinds of different AM fungus. The results showed that the biomass of No. 164 inoculated seedlings and the maximum leaf area of No. 171 inoculated seedlings were significantly higher than non-mycorrhizal seedlings. Compared with non-mycorrhizal seedlings, the plant height and root length of mycorrhizal seedlings were significantly reduced, while the root-crown ratio of mycorrhizal seedlings did not change significantly. The chlorophyll a/b value and carotenoid content of mycorrhizal seedlings were significantly higher than non-mycorrhizal seedlings, as was the total chlorophyll content, chlorophyll a content and chlorophyll h content of Gin01, Gin02 and No. 186 inoculated seedlings. Effects of AM fungus inoculation on the chlorophyll fluorescence characteristics of tobacco seedlings were larger. The effective PSⅡ quantum yield Y（Ⅱ）, the coefficients of photochemical quenching qP, relative maximal electron transport rate rETRmax, and onset of light saturation Ik of mycorrhizal seedlings were higher than that of the non-mycorrhizal seedlings, but the quantum yield of regulated non-photochemical energy loss in PS Ⅱ Y （NPQ） and quantum yield of non-regulated non- photochemical energy loss in PSⅡ Y（NO） were reduced. It is deduced that mycorrhizal seedlings was more tolerant to strong light and it will be able to make good use of the red light to promote growth of tobacco seedlings, but inoculation efficiency of different AM fungus is different.