金纳米棒荧光探针应用于多酚化合物检测新方法

Determination of Polyphenolic Compounds Based on Gold Nanorods by Fluorescence Analysis

以金纳米棒为荧光探针,在20%甲醇(pH 5.0～6.0)介质中,以植物多酚化合物芒果苷、瑞香素和白藜芦醇为检测对象,建立了3种植物多酚的灵敏、简便、快速检测新方法。多酚化合物基于其与金纳米棒表面的十六烷基三甲基溴化铵(CTAB)分子间的疏水作用而在金纳米棒表面富集,同时使金纳米棒在719 nm处的荧光强度减弱,在一定范围内多酚化合物的浓度与金纳米棒荧光强度成正比,其检出限分别为5.0×10-8、8.0×10-8、2.0×10-7mol/L。

Cetyltrimethyl ammonium bromide（CTAB） was attached on gold nanorods（AuNRs） as a stabilizing agent,which can enrich the plant polyphenolic compounds（HQ） on the surface of gold nanorods and lead to weaken the fluorescent intensity of gold nanorods by hydrophobic effect.When different polyphenolic compounds were added to gold nanorods system,the fluorescent intensity of AuNRs in 719 nm was decreased significantly.Under the experimental conditions of 20% methanol（pH 5.0-6.0）,the fluorescent intensity of AuNRs as fluorescent probe was linearly related to the concentration of three polyphenolic compounds（mangiferin,daphnetin and resveratrol）.In this way,a new method for the analysis of three polyphenolic compounds with sensitivity,simplity and quickness was established.The detection limits of three polyphenolic compounds were 5.0×10^-8,8.0 ×10^-8,2.0 ×10^-7mol·L^-1,respectively.