管碟法比较盐酸小檗碱和黄连药材对金色葡萄球菌的抑菌效力

Comparison of Antimicrobial Potency between Berberine Hydrochloride and Drug Material Coptis Chinensis on Staphyloccocus Aureus by Cup-Plate Method

目的建立比较盐酸小檗碱和黄连药材对金色葡萄球菌抑菌效力的方法。方法以盐酸小檗碱为参照物、抑菌圈直径(D)为反应值,按2010年版《中国药典(二部)》附录ⅪA抗生素生物检定法管碟法的三剂量法设计试验,对盐酸小檗碱和黄连药材样品的抑菌效力进行比较。结果盐酸小檗碱质量浓度在4.00～12.5μg/mL范围内,黄连样品溶液质量浓度在66.0～200μg/mL范围内,其剂量的对数值与抑菌圈直径呈良好的线性关系(r均大于0.99)。三剂量法剂间变异分析及可靠性测验结果表明,回归非常显著(P<0.01),偏离平行、二次曲线、反二次曲线均不显著(P>0.05),实验结果成立;组内(碟间)差异非常显著(P<0.01);平均可信限为1.8845%。结论该方法能用于比较盐酸小檗碱和黄连药材的抑菌效力。

Objective To establish the cup plate method for comparing the antimicrobial potency of berberine hydrochloride and Coptis chinensis on Staphyloccocus aureus. Methods With berberine hydrochlo.ride as the reference substance and the inhibition zone diameter （D） as the response value, the experiment was designed according to the antibiotic bioassay cup plate method in the appendix X]A of the Chinese Pharmacopoeia（edition 2010, Part 2）. The antimicrobial potency of berberine hydrochloride and the sample of medicinal ma terial Coptis chinensis was detected and compared. Results In the mass concentration ranges of 4. 00 12.5 μg/mL for berberine hy drochloride and 66.0 200 μg/mL for Coptis chinensis sample, the dose logarithm value respectively demonstrated better linear relation with the inhibition zone diameter （r 〉 0.99）. The variation analysis between doses in the 3 dose method and the reliability test results showed that regression was very significant （P 〈0.01） and the deviation of parallelism, second degree curve, anti second degree curve were not significant（P 〉0.05）. The experimental results were established. The difference within groups was very significant （P 〈0.01）. The average confidence limit was 1. gg4 5%. Conclusion This biological assay method could be used for testing the antimicrobial po tency of berberine hydrochloride and Coptis Chinensis.