摘要
目的建立测定五明丹胶囊中丹参酮ⅡA含量的高效液相色谱法。方法采用Eclipse XDB-C18色谱柱(150 mm×4.6 mm,5μm),甲醇-水(75∶25)为流动相,流速为1.0 mL/min,检测波长为270 nm。结果丹参酮ⅡA进样量在17.2～154.8μg范围内与峰面积线性关系良好(r=0.999 8),平均回收率为98.74%,RSD=1.22%(n=9)。结论该方法操作简便,专属性强,结果准确,可用于五明丹胶囊的质量控制。
Objective To establish an HPLC method to determining the Tanshinone ⅡA content in Wumingdan Capsules. Methods The chromatographic system consisted of Eclipse XDB C18 column, using methanol water（75：25） as the mobile phase. The detection wave length was 270 nm and the flow rate was 1.0 mL/min. Results The sample size of tanshinone ⅡA in the range of 17.2 154.8 μg had a good linear relation with the peak area（ r =0. 999 8）. The average recovery rate was 98.74%, RSD = 1.22% （n =9）. Conclusion The established method is simple, highly sensitive and accuracy, which can be used for the quality control of Wumingdan Capsules.
出处
《中国药业》
CAS
2012年第19期40-41,共2页
China Pharmaceuticals
