镜鲤酸性磷酸酶的QTL分析

Quantitative Trait Loci Analysis for Acid Phosphatase in Mirror Carp

利用992个微卫星标记检测了德国镜鲤(Cyprinus carpio)F1代190个个体基因组DNA进行基因型检测,共组成51个连锁群,覆盖基因组总长度为5138.2cM,标记间平均距离为5.19cM;利用软件MapQTL 6.0采用区间作图法对酸性磷酸酶性状进行数量性状基因座(QTL)定位分析。研究结果共检测到9个与酸性磷酸酶有关的QTLs,分布于8个连锁群。其中LG24连锁群LOD值最大(5.37),位于LG24连锁群,最大的可解释表型变异为13.8%。通过BLAST与斑马鱼进行序列比对,找到了与斑马鱼富含亮氨酸重复蛋白、横跨膜蛋白50a、ADP核糖化因子和细胞因子受体家族b8同源的分子标记。本研究结果对鲤鱼分子标记辅助育种和疾病调控具有重要应用价值。

In this study,992 microsatellite markers were selected to analyze the genomic DNA of 190 individuals derived from the sibling group of F1 hybrids mirror carp（Cyprinus carpio L.） The map was found to span 5138.2 cM over 51 linkage groups,with an average spacing of 5.19 cM.Then quantitative trait loci（QTLs） associated with Acid phosphatase（ACP） were identified by the interval mapping of the software MapQTL 6.0.Nine QTLs were identified for ACP on eight linkage groups.Among the nine QTLs controlling ACP in mirror carp,the maximum LOD score was found to be 5.37（LG24） in which 13.8% of the phenotypic variations were explained.By BLAST with the zebrafish for sequence alignment,molecular markers were found homologous with leucine-rich repeat-containing protein,transmembrane protein 50A,ADP-ribosylation factor and cytokine receptor family member b8 in zebrafish.The findings could be well used for molecular marker assisted selection and for disease control in mirror carp.