表皮生长因子受体和血管内皮生长因子与环氧化酶2在食管癌组织中的表达及其意义

Expression and significance of epidermal growth factor receptor and vascular endothelial growth factors andcyclooxygenase-2 in esophageal carcinoma

目的研究食管癌中表皮生长因子受体（EGFR）、血管内皮生长因子（VEGF）、环氧化酶2（COX-2）的表达及其与肿瘤组织病理特征之间的关系。方法应用免疫组化的方法分别检测94例食管癌组织和30例邻近正常食管黏膜中EGFR、VEGF蛋白、COX-2蛋白表达水平，并分析三者与肿瘤组织病理特征之间的关系。结果EGFR、VEGF、COXd在94例食管癌组织中阳性表达率分别为69．2％（65／94）、73．4％（69／94）、84．0％（79／94）；而在30例正常食管黏膜中阳性表达率分别为13．3％（4／30）、13．3％（4／30）、3．3％（1／30），2组间表达差异有统计学意义（P〈0．01）；且EGFR、VEGF、COX-2与淋巴结转移和TNM分期有关（P〈0．05）。结论EGFR、VEGF、COXd在食管癌组织中显著表达，且可能参与肿瘤发生、发展，可作为预测食管癌生物学行为的重要参考指标。

Objective To investigate the expression of epidermal growth factor receptor （EGFR）, vascular endothelial growth factor （VEGF） and cyclooxygenase-2 （COX-2） in esophageal carcinoma （EC） and their relationships with clinicopathologic features. Methods Using immunohistochemical methods, we assessed the expression levels of EGFR, VEGF and COX-2 in 94 surgically resected specimens from patients with EC and 30 normal contiguous esophageal mucosa specimens. We also explored the relationship between the histopathologic features and the expression levels of EGFR, VEGF and COX-2. Results The positive rates of EGFR, VEGF and COX-2 expressions were 13.3% （4/30）, 13.3% （4/30）, 3.3% （1/30）in normal mucosa and 69. 2% （65/94）, 73.4% （69/94）, 84.0% （79/94） in EC respectively. There were significant differences between the two groups （P 〈 0. 01 ）. In EC, high EGFR, VEGF and COX-2 expressions had a positive correlation with tumor node metastases classification and lymph node metastasis （P 〈 0.05 ）. Conclusions EGFR, VEGF and COX-2 are overexpressed in EC. They might participate in tumorigenesis and progression. Over-expression of EGFR, VEGF and COX-2 can be used as a biological marker to evaluate the behavior of EC.