牙周炎对慢性阻塞性肺疾病大鼠肺损伤的作用研究

Investigation of the pathogenesis of lung injury in rats with chronic obstructive pulmonary diseases effected by chronic periodontitis

目的:应用SD大鼠建立慢性牙周炎(chronic periodontitis,CP)和慢性阻塞性肺疾病(chronic obstructive pulmonarydiseases,COPD)动物模型,探讨CP在COPD发生发展过程中对肺损伤的作用。方法:将40只SD大鼠雌雄各半随机分成4组:正常对照组,CP组,COPD组,COPD伴CP组,每组10只。各组按照实验设计施加相应建模因素,实验10周末分别检查大鼠的龈沟出血指数(SBI)、肺功能状况,ELISA法检测血清中TNF-α和IL-1含量,然后处死大鼠,取材观察牙槽骨吸收程度(ABL)、牙周组织和肺部组织病理学改变,对数据进行统计分析。结果:COPD伴CP组大鼠的肺功能明显降低,龈沟出血指数和牙周组织改变与CP组相似,实验组血清IL-1和TNF-α水平明显高于对照组(P<0.05)。结论:CP在COPD大鼠肺损伤的发生发展过程中具有促进作用。

Objective： To investigate the potential correlation between chronic periodontitis （CP） and chronic obstructive pulmonary diseases（COPD） in SD rats. Methods： Equal genders of SD rats were randomly divided into 4 groups： control group, CP group, COPD group and COPD with CP group（ n = 10）. The rats in each group were imposed into the predesigned appropriate intervention for the construction of disease models. 10 weeks after the set-up of the disease models, pulmary function was examined, serum TNF-α and IL-1 were detected by ELISA, sulcus bleeding index（SBI） was observed. Then the animals were sacrificed. The level of alveolar bone loss（ABL） and the bisto-pathological changes of periodontal tissue and lung tissue were evaluated. Data were analysed statistically. Results： In CP group and COPD with CP group SBI and ABL were more than in control group and COPD group（ P 〈 0.05 ）. Pulmonary function in group COPD with CP was worse than that in group COPD. The levels of serum TNF- α and IL- 1 increased in the test groups compared with those in control group, the greatest change was found in group D（P 〈0.05）. Conclusion： CP may be a risk factor of lung injury in COPD.