Beclin 1和LC3在腮腺多形性腺瘤及癌在多形性腺瘤中的表达和意义

Expression and significance of Beclin1 and LC3 in parotid pleomorphic adenoma and carcinoma in pleomorphic adenoma

目的:探讨自噬相关基因Beclin1和微管相关蛋白LC3表达和腮腺肿瘤之间的关系。方法:用免疫组化方法检测20例正常腮腺组织、37例腮腺多形性腺瘤、41例癌在多形性腺瘤中Beclin1和LC3的表达,并结合临床病理因素进行分析。结果:Beclin1在正常腮腺组织、腮腺多形性腺瘤、癌在多形性腺瘤中的阳性率分别为100%、92%、63%;LC3在这3种组织的阳性表达率分别为100%、86%、56%。淋巴结转移及TNM分期与Beclin1的表达间有显著性关系(P<0.05),不同侵袭性、有无淋巴结转移与LC3的表达间有显著性关系(P<0.05)。结论:Beclin1和LC3在腮腺多形性腺瘤及癌在多形性腺瘤中表达下调,自噬功能改变可能在腮腺肿瘤形成中具有一定作用。

Objective： To investigate the expression of microtubule-associated protein light 1 chain 3 （LC3） and Beclinl in parotid tumors. Methods： The expression of Beelinl and LC3 was assessed by immunohistochemistry in 20 cases of normal parotid gland （ NPG）, 37 cases of parotid pleomorphic adenoma（PPA） and 41 cases of carcinoma in pleomorphic adenoma（CPA）. The correlations of Beclinl and LC3 expression to the clinicopathologic characteristics of CPA were analyzed. Results： The positive rates of Beclinl in NPC, PPA and CPA were 100% ,92% and 63% respectively, and those of LC3 were 100% , 86% and 56% respectively. In CPA, the expression of Beclinl was associated with lymph node metastasis and TNM stage（ P 〈 0.05 ） , the invasive of the tumor and lymph node metastasis was associated with the LC3 expression（ P 〈 0.05 ）. Conclusion ： The expressions of LC3 and Beclinl are down-regulated in PPA and CPA. The changes of autophagic capacity may be related to the development of.parotid gland tumors.