大豆异黄酮对壬基酚所致大鼠血清性激素紊乱及前列腺增生的改善作用

Improvement Effect of Soy Isoflavones(SI) on Serum Hormone Disorders and Benign Prostatic Hyperplasia(BPH) Induced by Nonylphenol(NP) in Rat

目的探讨壬基酚(Nonylphenol,NP)对大鼠前列腺脏器系数、组织病理学改变及对血清性激素水平的影响及大豆异黄酮(soybean isoflavones,SI)的保护效应。方法 50只健康雄性清洁级Wistar大鼠,随机分为5组,分别为橄榄油溶剂对照组,25.0mg/kg NP组,25.0mg/kg NP+50.0mg/kg SI组,25.0mg/kg NP+100.0mg/kg SI组,100.0mg/kg SI组。NP采用皮下注射染毒,SI采用灌胃染毒,染毒容积皆为1mL/kg,1次/d,连续30d。观察大鼠前列腺的湿质量、前列腺指数、组织病理学及血清性激素水平。结果 NP染毒组前列腺前叶质量、脏器指数高于溶剂对照组,差别有统计学意义(P<0.05);25.0mg/kg NP组、各NP+SI染毒组及100.0mg/kgSI组血清E2水平显著低于溶剂对照组(P<0.05);血清T水平除100.0mg/kg SI组外,余各组均高于溶剂对照组(P<0.05),E2/T比值低于溶剂对照组(P<0.05);与25.0mg/kg NP组比较,NP+SI染毒组前列腺前叶质量、脏器指数均低于单独染NP组,血清E2和E2/T比值高于该组(P<0.05)。组织病理学观察:NP染毒组大鼠前列腺上皮增生广泛,腺体密集,腺上皮细胞肥大,核增大,腔内嗜酸性分泌物较多;联合染毒组前列腺腺腔较规则平整,腔内嗜酸性分泌物量较少。结论 SI具有拮抗NP致雄性大鼠前列腺增生作用,这可能与其调节NP所致大鼠体内性激素水平紊乱有关。

Objective To study the effect of nonylphenol（NP） on rats’ prostatic index,histopathologic changes and the levels of sexual hormones level in serum;at the same time,to investigate whether soybean isoflavones（SI） has protective effect for NP’s.MethodsIn this study,we exposed fifty healthy Wistar rats to 25.0 mg/kg NP,25.0 mg/kg NP＋50.0 mg/kg SI,25.0 mg/kg NP ＋100.0 mg/kg SI,100.0 mg/kg SI in vitro for 30 days,the control group exposed to olive.After the treatment,the prostate wet weight,prostatic index and morphological changes and the serum hormones were observed.ResultsThe anterior lobe wet weight of prostate and organ coefficient in rats in group 25.0 mg/kg NP were significantly higher than those in olive group;and the anterior lobe wet weight and prostatic index in 25.0 mg/kg NP＋50.0 mg/kg,25.0 mg/kg NP ＋100.0 mg/kg SI were significantly lower than those in 25.0 mg/kg NP group（P〈0.05）;The serum E2 levels in each treatment group were lower than those in the olive group（P〈0.05 or P〈0.01）;the serum testosterone（T） and E2/T in all exposed groups（except group 100 mg/kg SI）were significantly higher or lower respectively than that in the olive group（P〈0.05 or P〈0.01）;the level of E2 and E2/T ingroup 25.0 mg/kg NP＋50.0 mg/kg and 25.0 mg/kg NP ＋100.0mg/kg SI were significantly higher than those in the group of 25.0 mg/kg NP（P〈0.05）;In NP group,we saw Prostatic hyperoplasia,although the glandular epithelial were rule and cavity acid secretion was less in of NP＋SI group compared with 25.0 mg/kg NP group.ConclusionsThese results suggest Soybean isoflavones have significant antagonism effect on prostatic hyperplasia which caused by nonylphenol through regulated sex hormone levels in rats.