间日疟现症患者外周血树突状细胞亚群的变化

Changes of dendritic cells and its subsets in peripheral blood of patients with vivax malaria

目的:观察间日疟现症患者外周树突状细胞(dendritic cells,DC)亚群比例的的变化,探讨DC亚群在间日疟发病机制中的作用。方法:采用流式细胞术四色荧光分析法检测18例间日疟现症患者(疟疾组)和36名健康成人(对照组)外周血的浆细胞样DC(pDC)和髓样DC(mDC)的比例。结果:疟疾组患者pDC明显低于对照组(P<0.01),mDC/pDC明显高于对照组(P<0.01),而mDC在2组间无明显不同(P>0.05)。结论:间日疟现症患者机体呈现免疫抑制状态,间日疟原虫与DC相互作用,可能通过调整mDC/pDC的比值参与间日疟感染的Th1型免疫应答的诱导和调控。

Objective：To analyze the changes of dendritic cells （DC） and its subsets in peripheral blood of patients with vivaxmalaria, and explore its immune mechanism. Methods： Eighteen patients with vivax malaria （ malaria group） and 36 healthy adults （ control group） were selected. The proportions of plasmacytoid DC （ pDC ） and myeloid DC （ mDC ） in peripheral blood were detected by flow cytometry four-colour fluorimetric analysis technique. Results：pDC subset in malaria group was significantly lower than that in control group（ P 〈 0.01 ）. mDC/pDC in malaria group was significantly higher than that in control group （P 〈 0.01 ）, and there was no difference in the proportion of mDC between the two groups （ P 〉 0.05 ）. Conclusions： There presents a general immunosuppression during plasmodium vivax infections. Plasmodium vivax parasites interact with DC, which involves inducing and adjusting Thl immune response by altering mDC/pDC ratio.