酵母展示脂肪酶催化合成葡萄酒香料己酸丁酯

Catalytic Synthesis of Butyl Hexanoate by Using Lipase B Displayed on the Surface of Candida Antarctica

以毕赤酵母展示的南极假丝酵母脂肪酶B为催化剂,己酸和丁醇为反应底物,合成葡萄酒用香料己酸丁酯。结果表明,酵母展示脂肪酶是合成己酸丁酯的良好催化剂,其最适催化工艺条件为:酸醇物质的量比为1:1.2,催化剂展示脂肪酶用量为2%,反应时间为4 h,酯化率可达97.2%。

Lipase B displayed on the surface of Candida antarctica was used as catalyst with hexanoic acid and butyl alcohol as reaction substrate to synthesize butyl hexanoate. The experimental results suggested that yeast surface-displayed lipase was a good catalyst and the optimum technical conditions were summed up as follows： molar ratio ofhexanoic acid to butyl alcohol was 1：1.2, the use level of catalyst was 2 %, the reaction time was 4 h, and esterification rate reached up to about 97.2 %.