2型糖尿病患者CR1基因多态性与红细胞CR1数量、黏附活性相关性研究

Study on the correlation of CR1 gene polymorphism with erythrocyte CR1 expression and its adhesive activity in patients with type 2 diabetes mellitus

目的研究2型糖尿病(T2DM)患者1型补体受体(CR1)基因多态性与红细胞CR1数量、黏附活性的相关性。方法应用限制性内切酶HindⅢ聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术测定132例T2DM患者和124名正常健康人群CR1基因多态性,用酶联免疫吸附试验(ELISA)测定红细胞的CR1数量,检测红细胞黏附活性,并做统计学分析。结果 T2DM患者红细胞表面的CR1数量和黏附活性均低于正常对照组(P<0.01)。CR1基因型在2组的分布频率均为HH型最高、HL型次之、LL型最低。T2DM组CR1基因等位基因L频率为14.8%,高于对照组(11.3%),但2组CR1基因型频率和等位基因频率差异均无统计学意义(P>0.05)。2组组内比较,HH型与HL型红细胞CR1数量及黏附活性差异均无统计学意义(Ρ均>0.05)。T2DM组HH型与HL型红细胞CR1数量与黏附活性均低于正常对照组(Ρ<0.05)。结论 T2DM患者与正常对照者间CR1基因型频率和等位基因频率无差异,而T2DM患者红细胞CR1数量及黏附活性减低,提示T2DM的发生与红细胞免疫功能低下有关。

Objective To study on the correlation of complement receptor type 1 （ CR1 ） gene polymorphism with erythrocyte CR1 expression and its adhesive activity in patients with type 2 diabetes mellitus （T2DM）. Methods A total of 132 patients with T2DM were determined for the CR1 gene polymorphism by restriction incision enzyme Hind III polymerase chain reaction-restriction fragment length polymorphism （ PCR-RFLP）, and enzyme-linked immunosorbent assay （ELISA） was performed to detect the expression of CR1 on erythrocyte and its adhesive activity. Meanwhile, 124 healthy subjects were enrolled as controls. The results were analyzed statistically. Results The expression of CR1 on erythrocyte and its adhesive activity in patients with T2DM were lower than those in healthy controls （P 〈 0.01 ）. The CR1 genotype distribution frequencies from high to low were type HH, type HL and type LL by turns. The allele L frequency of CR1 gene in T2DM patients （ 14.8% ） was higher than that in healthy controls （ 11.3% ） , and there was no statistical significance in CR1 genotype frequency and allele frequency between the 2 groups （P 〉 0. 05 ）. The expression of CR1 and adhesive activity of type HH and type HL had no statistical significance （ P 〉 0.05 ）. The expression of CR1 and adhesive activity of type HH and type HL in T2DM patients were significantly lower than those in healthy controls （P 〈 0. 05 ）. Conclusions The genotype and allele frequencies in patients with T2DM have no statistical difference with those in healthy controls,but the expression of CR1 on etythrocyte and the adhesive activity in patients with T2DM decrease significantly. It indicates that the decrease of erythrocyte CR1 adhesive activity may be attribute to the occurrence of T2DM.