摘要
目的探讨冠心病患者血小板膜上蛋白酪氨酸磷酸酶(CD148)的变化。方法采用流式细胞仪三色免疫荧光分析检测健康老年人和冠心病患者血小板膜上蛋白酪氨酸磷酸酶(CD148)的阳性百分率、蛋白酪氨酸磷酸酶(CD148)的几何荧光强度、P-选择素(CD62P)的阳性百分率、P-选择素(CD62P)的几何荧光强度及膜糖蛋白Ⅱb/Ⅲa复合物(PAC-1)的阳性百分率、膜糖蛋白Ⅱb/Ⅲa复合物(PAC-1)的几何荧光强度的表达水平。结果冠心病患者和健康老年人的血小板膜CD62P、CD62P几何荧光强度表达量相比较(6.24 vs 0.89,P<0.01;45.64 vs 25.38,P<0.05),差异有统计学意义;CD148、CD148几何荧光强度表达量相比较(7.12 vs 0.45,P<0.01;41.35 vs 20.12,P<0.01),差异有统计学意义;PAC-1及PAC-1几何荧光强度的表达量(22.64 vs 1.84,P<0.01;43.46 vs 22.45,P<0.01),差异有统计学意义,但与当日血压、血糖及血脂无相关关系。结论 CD62P及PAC-1、CD148是血小板活化的敏感指标,冠心病患者有血小板活化现象,为冠心病患者提供了理论检测指标。
Objective To investigate the changes in tyrosine phosphatase（CD148） ,P selection （CD62P） and glycolprotein Ⅱ b/Ⅲa（ PAC 1 ） in platelets of elderly patients with eoronary heart disease （CHD）. Methods CD148, CD62P and PAC 1 in platelets were analyzed by flow cytometric 3 color immunofluorescence. Results The percentage and MFI of CD62 expression in the platelet membrane in elderly patients with coronary heart disease（CHD） were increased compared with healthy individuals（6.24 vs O. 89 ,P 〈 0.01 ;45.64 vs 25.38 ,P 〈 0.05） , so did the percentage and MFI of CD148 expression in the platelet membrane in elderly pa- tients with type 2 diabetes （7.12 vs O. 45 ,P 〈0.01 ;41.35 vs 20.12 ,P 〈0.01 ）. The percentage of PAC - 1 and MFI expressed in the platelct membrane also increased （ 22.64 vs 1.84, P 〈 O. 01 ;43.46 vs 22.45, P 〈 O. 01 ） , but not related to blood pressure or the con tent of blood lipid and gluocse. Conclusion CD148, CD62P and PAC - 1 can be regarded as sensitive and specific molceular markers for activated platelets.
出处
《武警医学》
CAS
2012年第9期763-766,共4页
Medical Journal of the Chinese People's Armed Police Force
