辣椒素/SiO_2微球的制备

Preparation of Capsaicin/SiO_2 microspheres and the optimization of its encapsulation efficiency

【目的】制备辣椒素/SiO2微球,并对其制备参数进行优化。【方法】以油包水包油(O/W/O)复乳法制备辣椒素/SiO2微球,采用扫描电镜和光学显微镜检测微球形态和粒径,紫外分光光度法测定微球包封率。通过L9(34)正交试验,对外油相稳定剂羟丙基纤维素(HPC)、水相稳定剂聚乙二醇(PEG)、亲水性表面活性剂Tween 20和亲油性表面活性剂Span 80的用量进行优化。【结果】用O/W/O复乳法制得了具有中空结构的辣椒素/SiO2微球,微球平均粒径(4.62±0.12)μm,分散系数ε为0.76。在不考虑交互作用的条件下,优选出较高包封率的微球制备参数组合为:HPC与Span 80含量分别为外油相质量的1.5%和0.5%,PEG与Tween 20含量分别为水相质量的4.0%和0.5%。【结论】优选出了包封率较高的辣椒素/SiO2微球制备参数,成功制备了辣椒素/SiO2微球。

【Objective】 The study was conducted to prepare Capsaicin/SiO2 microspheres,and to optimize their preparation parameters.【Method】 Capsaicin/SiO2 microspheres were prepared by O/W/O multiple emulsion,and the encapsulation efficiency was detected by UV spectrophotometry.The preparation parameters,such as amount of the external oil stabilizer HPC,water phase stabilizer PEG,hydrophilic surfactant Tween 20 and lipophilic surfactant Span 80 were optimized by L9（34）orthogonal method according to the Capsaicin encapsulation efficiency.The morphology and size of the microspheres were characterized by SEM and optical microscopy.【Result】 Spherical hollow Capsaicin/SiO2 microspheres were prepared by O/W/O multiple emulsion.The average diameter of the microsphere was about（4.62±0.12） μm,and the dispersion coefficient（ε）was ca.0.76.If there was no cross-interaction,the optimal process parameters for high encapsulation efficiency should be：HPC and Span 80 contents should be 1.5% and 0.5% respectively of the external oil phase,and PEG and Tween 20 content should be 4.0% and 0.5% respectively of the water phase.【Conclusion】 The process parameters were optimized,by which Capsaicin/SiO2 microspheres with comparatively high encapsulation efficiency can be prepared.