摘要
在pH=7.40的Tris-HCl缓冲溶液中,采用荧光光谱法和粘度法研究了8-羟基喹啉(HQ)与鲱鱼精DNA的作用方式.用摩尔比法确定了HQ与DNA的结合比为12∶1.通过热力学研究得出,在27℃时HQ与DNA之间相互作用的Kφ27℃=3.844×105L/mol,热力学函数ΔrHφm=-5.08×104J/mol,ΔrGφm 300.15K=-3.2×104J/mol,ΔrSφm=-62.6 J/(mol.K).结果显示该反应为焓驱动.实验表明HQ与DNA的作用方式为部分嵌插与静电作用方式.
The mechanism of interaction between HQ and DNA has been studies by fluorescence spectra and viscosi- ty measurements in solution of Tris - HC1 （ pH = 7.4）. From mole ration method, the mole ration between HQ and DNA is 12： 1. Through the study of the thermodynamic we known that binding constant of HQ interaction with DNA is K25℃ = 3. 844 × 105 L/mol, the corresponding thermodynamic parameters are △rHm ： - 5. 08 × 104 J/mol, △rGm300.15K =-3.2 × 104 J/mol, △rSm = -62.6 J/（mol · K）. The result suggested that enthalpy was the driven force in this reaction. And the interaction of HQ and DNA through by electrostatic interaction and partial intercalation method.
出处
《商丘师范学院学报》
CAS
2012年第9期49-52,共4页
Journal of Shangqiu Normal University
基金
国家自然科学基金资助项目(30973634)
关键词
鲱鱼精DNA
8-羟基喹啉
溴化乙锭
荧光光谱
作用方式
herring sperm DNA
8 - Hydroxyquiuoline
ethidium bromide
fluorescence spectra
interaction method
