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全血DNA提取方法的改进及PCR检测 被引量:6

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摘要 采用TritonX-100和SDS破碎红细胞和白细胞,氯仿抽提,无需苯酚和蛋白酶K,在30 min内可提取一定量的基因组DNA。结果显示,DNA纯度约1.8;电泳检测条带清晰;使用PCR扩增评价提取核酸的质量,得到预期的目的条带。说明该方法具有安全、快速、经济的特点,是一种理想的提取血液基因组DNA的方法。
机构地区 河南科技学院
出处 《江苏农业科学》 CSCD 北大核心 2012年第8期56-57,共2页 Jiangsu Agricultural Sciences
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