Ⅱ型抗癌晶体蛋白的芳香族氨基酸及其与抗肝癌活性间的关系

Relationship Between Aromatic Amino Acids of Parasporin-2 and its Anti-Liver-Cancer Activity

利用氨基酸序列比对,蛋白质间相互作用位点预测和蛋白质与蛋白质对接,研究Ⅱ型抗癌晶体蛋白的氨基酸组成与其抗肝癌活性之间关系.结果表明:Ⅱ型抗癌晶体蛋白分子上位点49,51,52,55～60,194和205～212的氨基酸残基,特别是芳香族氨基酸在配体和受体蛋白质间的相互作用中起着重要作用;在Hex的蛋白质与蛋白质对接中,配体P11和P32与受体甘油醛-3-磷酸脱氢酶(GAPDH)的结合能量最低,表明P11和P32更容易与GAPDH结合.

In this study, amino sequences blast, protein-protein interaction sites prediction and protein：protein docking were employed to explore the relationship between amino acid composition of parasporin-2 and its anti-liver-cancer activity. The results showed that amino residues, especially the aromatic amino residues at sites 49, 51, 52, 55-60, 194 and 205-212 in parasporin-2 were found to play a key role in ligand-reeeptor interaction. Protein-protein docking on Hex illustrated that ligands Pll and P32 had lower total binding energy value than the others when glyceraldehyde-3-phosphate dehydrogenase （GAPDH） was the receptor, indicated that Pll and P32 were easier to bind to GAPDH.