全自动血培养系统在外伤性眼内炎的临床应用

Clinical application of automated blood culture system in traumatic endophthalmitis

目的评价全自动血培养系统（BacT／ALERT3D）在外伤性眼内炎的临床应用。方法外伤性眼内炎113例（113眼）的玻璃体液进行病原菌培养，用中和抗生素儿童培养瓶（BacT／A-LERTPF）采集标本，全自动血培养系统进行病原菌培养，Vitek-2compact进行菌种鉴定和药敏试验，对检出阳性者，分析阳性率和病原菌种类。结果113份玻璃体液标本检出病原微生物81例，阳性率为71．7％，其中革兰阳性细菌占62．9％，革兰阴性细菌占34．6％，真菌占2．5％。最快检出时间为4h。结论全自动血培养系统应用于外伤性眼内炎提高了玻璃体液病原菌培养的阳性率，缩短阳性检出时间，检出病原菌种类多，结果准确。

Objective To evaluate the clinical application of BacT/ALERT 3D automated blood culture system in traumatic endophthalmitis. Methods A total of 113 vitreous specimens of traumatic en-dophthalmitis were collected by BacT/ALERT PF pediatric blood culture bottle, detected by BacT/ALERT 3 D automated blood culture system and analysed by Vitek -2 compact. We evaluated the positive rate, time to show positive, the types of microorganisms. Results In a total of 113 cultured vitreous specimens, 81 cases were positive. Positive rate was 71.7%. Grampesitive microorganisms accounted for 62.9% of the positive culture results. Gramnegative microorganisms accounted for 34.6% and fungi accounted for 2.5%. The shortest time to show positive was 4h. Conclusion The automated blood culture system has been wide-ly used in blood and body fluid culture. The application of the automated blood culture for endophthalmitis can raise the positive detection rate, reduce the detection time to show positive and increase the types of mi-croorganisms detected.