大鼠骨髓间充质干细胞体外培养

Rats Bone Marrow Mesenchymal Stem Cells in Vitro

[目的]通过实验探索并验证行之有效的体外骨髓间充质干细胞分离、纯化、培养的方法。[方法]采用密度梯度离心法和贴壁培养法相结合的方法对骨髓间充质干细胞进行体外培养。[结果]原代细胞10d左右达85%以上融合,传代后细胞约1周左右达85%以上融合;传至5代细胞形态呈较一致的长梭形。[结论]运用贴壁培养法和密度梯度离心法相结合的方法对骨髓间充质干细胞进行体外培养,可获得足够数量纯度较高的骨髓间充质干细胞。

[ Objective ] According to the characteristics of various methods, combining research needs, our investigation aimed to explore and verify an effective separation, purification and amplification methods in vitro BMSCs. [ Methods ] BMSCs was cultured in vitro by density gradient centrifugation method combined with adhesive method. [ Results ] Primary cells fusion was up to over 85 % , cells fusion after subculturing was up to over 85% after about 1 weeks; Ceils passaged to 5 thgeneration presented consistent fusiformis. [ Con- clusion ] The experiment selected CD34, CD44, CD45 and CD90 to identify and testify the BMSCs cultured. Adherent screening method and density gradient centrifugation methods were used to obtain enough and quantity of BMSCs, which laid the foundation for the next step experiments in vivo.