穴位注射对非酒精性脂肪肝大鼠肝功能及脂质代谢的影响

Point Injection on Liver Function and Lipid Metabolism of Nonalcoholic Fatty Liver Rats

目的探讨足三里穴位注射硫普罗宁治疗非酒精性脂肪肝的作用机制。方法将32只SD大鼠随机分为正常组、模型组、腹腔注射组、穴位注射组,除正常组外,剩余24只大鼠用高脂饲料诱导大鼠高脂性脂肪肝模型。造模成功后,腹腔注射组、穴位注射组按80 mg/(kg.d)予腹腔内及足三里注射硫普罗宁,治疗6周。正常组、模型组不做任何处理。第6周末处死全部大鼠。检测各组大鼠血清谷氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)活性,采用CHOD-PAP法测定血清和肝组织胆固醇(TC),采用GPO-PAP法测定血清和肝组织三酰甘油(TG)。结果模型组血清AST、ALT、TC、TG明显升高(P<0.01),肝组织TC、TG升高(P<0.05);腹腔注射组治疗后与模型组比较差异无统计学意义;与模型组、腹腔注射组比较,穴位注射组能明显改善脂肪变性,降低肝脏炎症反应,降低大鼠TG、TC、ALT、AST水平(P<0.05)。结论足三里穴位注射硫普罗宁能明显非酒精性脂肪肝大鼠降低血脂、肝脂,保护肝细胞。

Objective To explore the mechanism of Zusanli point injection tiopronin treatment for nonalcoholic fatty liver. Methods Thirty-two SD rats were randomly divided into normal group, model group, intraperitoneal injection group, point injection group. Except the normal group, the remaining 24 rats were induced fatty liver model with high fat diet. After modeling, intraperitoneal injection group was given intraperitoneal injection of tiopronin 80 mg/（kg·d）, point injection group was given Zusanli injection tiopronin for 6 weeks. Normal group and model group received no treatment. All rats were killed in the sixth week. Serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST） were measured. Cholesterol （TC） and triglyceride （TG） of serum and liver tissue were measured by the GPO-PAP and CHOD-PAP method. Results Serum AST, ALT, TC, TG of model group were significantly higher （P〈0.01）, liver tissue TC, TG increased （P 〈0.05）. After treatment, intraperitoneal injection group has no significant difference compared with model group. Compared with model group and intraperitoneal injection group, point injection group significantly improved steatosis, decreased liver inflammation, reduced TG, TC, ALT, AST level （P〈0.05）. Conclusion Zusanli point injection tiopronin can lower liver fat and protect liver cells of nonalcoholic fatty liver rats.