仙人掌多糖对大鼠局灶性脑缺血的神经保护作用

Neuroprotective Effect of Cactus Polysaccharides on Focal Cerebral Ischemia in Rats

目的观察仙人掌多糖对大脑中动脉栓塞(MCAO)诱导的大鼠缺血-再灌注损伤的神经保护作用。方法雄性SD大鼠随机分为假手术组、缺血组、0.9%氯化钠溶液组、仙人掌多糖治疗组(200 mg·kg-1·d-1,ip)。采用MCAO法制作大鼠缺血-再灌注损伤模型。分别于大鼠缺血2 h再灌注3,6,8 h后进行神经行为学评分;8 h后2,3,5-三苯基氯化四氮唑(TTC)染色测定脑梗死体积;缺血2 h再灌注22 h后脑组织切片苏木精-伊红(HE)染色显微镜下形态学观察。结果与0.9%氯化钠溶液组相比,再灌注8 h后仙人掌多糖治疗组神经行为学评分平均下降(2.35±0.47)分(P<0.05),梗死灶体积减少(P<0.05);大鼠皮质及海马组织神经细胞丢失、神经胶质增生、核固缩、核深染等形态学均有明显改善(P<0.05)。结论仙人掌多糖可以缓解大鼠大脑中动脉栓塞症状,具有一定的神经保护作用。

Objective To study the neuroprotective effect of cactus polysaccharides（CP） on ischemia/reperfusion（I/R） injury induced by the middle cerebral artery occlusion（MCAO） in rats.Methods The male SD rats were randomly divided into sham-operated,MCAO/R model group,NS-treated group,CP-treated group（200 mg·kg-1·d-1,ip）.The MCAO/R model was made by MCAO method.The neurological deficits were detected at 3 h,6 h and 8 h after MCAO for 2 h and reperfusion;The cerebral infarct volume was detected by TTC staining 8 h later,the brain tissue sections of MCAO for 2 h and reperfusion for 22 h were stained by hematoxylin-eosin（H＆E） for the morphological observation under microscope.Results Compared to the NS-treated group,the neurological deficit scores of CP-treated group after reperfusion for 8 h was decreased by（2.35±0.47）（P〈0.05）,in company with the loss of infarct volume（P〈0.05）.The rat cortical and hippocampal neuronal cell loss,gliosis,nuclear condensation and nuclear deep dyeing were significantly alleviated（P〈0.05）.Conclusion The CP can attenuate of arterial embolism in rats and ameliorate cerebral ischemia,which possessing a neuroprotective effect.